{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:44:45Z","timestamp":1772160285139,"version":"3.50.1"},"reference-count":99,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T00:00:00Z","timestamp":1647561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","award":["RSG\\R1\\180213"],"award-info":[{"award-number":["RSG\\R1\\180213"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","award":["CHL\\R1\\180031"],"award-info":[{"award-number":["CHL\\R1\\180031"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evolutionary Applications"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Crop losses to plant pathogens are a growing threat to global food security and more effective control strategies are urgently required. Biofumigation, an agricultural technique where\n                    <jats:italic>Brassica<\/jats:italic>\n                    plant tissues are mulched into soils to release antimicrobial plant allelochemicals called isothiocyanates (ITCs), has been proposed as an environmentally friendly alternative to agrochemicals. Whilst biofumigation has been shown to suppress a range of plant pathogens, its effects on plant pathogenic bacteria remain largely unexplored. Here, we used a laboratory model system to compare the efficacy of different types of ITCs against\n                    <jats:italic>Ralstonia solanacearum<\/jats:italic>\n                    plant bacterial pathogen. Additionally, we evaluated the potential for ITC\u2010tolerance evolution under high, intermediate, and low transfer frequency ITC exposure treatments. We found that allyl\u2010ITC was the most efficient compound at suppressing\n                    <jats:italic>R<\/jats:italic>\n                    .\n                    <jats:italic>solanacearum<\/jats:italic>\n                    growth, and its efficacy was not improved when combined with other types of ITCs. Despite consistent pathogen growth suppression, ITC tolerance evolution was observed in the low transfer frequency exposure treatment, leading to cross\u2010tolerance to ampicillin beta\u2010lactam antibiotic. Mechanistically, tolerance was linked to insertion sequence movement at four positions in genes that were potentially associated with stress responses (H\u2010NS histone like protein), cell growth and competitiveness (acyltransferase), iron storage ([2\u2010Fe\u20102S]\u2010binding protein) and calcium ion sequestration (calcium\u2010binding protein). Interestingly, pathogen adaptation to the growth media also indirectly selected for increased ITC tolerance through potential adaptations linked with metabolism and antibiotic resistance (dehydrogenase\u2010like protein) and transmembrane protein movement (Tat pathway signal protein). Together, our results suggest that\n                    <jats:italic>R<\/jats:italic>\n                    .\n                    <jats:italic>solanacearum<\/jats:italic>\n                    can rapidly evolve tolerance to allyl\u2010ITC plant allelochemical which could constrain the long\u2010term efficiency of biofumigation biocontrol and potentially shape pathogen evolution with plants.\n                  <\/jats:p>","DOI":"10.1111\/eva.13363","type":"journal-article","created":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T22:08:39Z","timestamp":1645740519000},"page":"735-750","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Plant pathogenic bacterium can rapidly evolve tolerance to an antimicrobial plant allelochemical"],"prefix":"10.1111","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6262-2185","authenticated-orcid":false,"given":"Carrie Louise","family":"Alderley","sequence":"first","affiliation":[{"name":"Department of Biology University of York  York UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel Terrence Edwards","family":"Greenrod","sequence":"additional","affiliation":[{"name":"Department of Biology University of York  York UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ville\u2010Petri","family":"Friman","sequence":"additional","affiliation":[{"name":"Department of Biology University of York  York UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01416095"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw387"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/J.1365\u20102958.1997.3151679.X"},{"key":"e_1_2_6_5_1","unstructured":"Back M. Barker A. &Evans K.(2019).Effectiveness of biofumigant crops for the management of PCN in GB.Agricultural And Horticultural Development Board (AHDB). Available at:https:\/\/pure.sruc.ac.uk\/en\/publications\/effectiveness\u2010of\u2010biofumigant\u2010crops\u2010for\u2010the\u2010management\u2010of\u2010pcn\u2010in\u2010g(Accessed: 21 April 2021)"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1614\/WS\u201008\u2010151.1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1614\/WS\u2010D\u201010\u201000137.1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0038\u20100717(98)00163\u20101"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X16500245"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/BIOINFORMATICS\/BTU170"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf00055a033"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20101097(03)00882\u20106"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.00414"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.1997.87.3.250"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02854019"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01967\u201013"},{"key":"e_1_2_6_18_1","first-page":"9","volume-title":"\u2018The current bacterial wilt situation: a global overview\u2019. 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