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Murdock Charitable Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000937","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evolutionary Applications"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Island populations are at heightened risk of inbreeding due to reduced mating opportunities with unrelated conspecifics. Extensive inbreeding can result in inbreeding depression (reduced fitness of individuals with related parents). Alexander Archipelago wolves (<jats:styled-content style=\"fixed-case\"><jats:italic>Canis lupus ligoni<\/jats:italic><\/jats:styled-content>) are a geographically isolated subspecies that occur in the Southeast Alaskan panhandle, USA, and coastal British Columbia, Canada. Wolves on the Prince of Wales Island complex (POW) in Southeast Alaska are expected to have lower levels of resiliency because they are a small, insular population that has experienced habitat fragmentation and cycles of moderate to heavy harvest. To understand the extent of population structure and inbreeding in Alexander Archipelago wolves, we designed a DNA hybridization capture for wolves and sequenced captured DNA from 58 individuals sampled from across Southeast Alaska during 2002\u20132016. Estimates of the proportion of the genome in runs of homozygosity (<jats:italic>F<\/jats:italic><jats:sub>ROH<\/jats:sub>) regardless of run length, revealed that POW wolves were most inbred compared to wolves in other areas of Southeast Alaska. Wolves on POW also had more long (\u2265\u200910\u2009Mb) runs of homozygosity than the other populations we assessed, indicating more frequent mating between individuals with recent common ancestors (1\u201310 generations ago). This pattern indicates a smaller population size for POW wolves in the recent past compared to other Southeast Alaskan populations. Wolves on POW exhibit an extent of inbreeding similar to that observed in Isle Royale National Park wolves, a population that has exhibited severe inbreeding depression. Our work demonstrates the utility of using genomic capture data to infer individual inbreeding so that proactive management (e.g., setting population targets and harvest quotas, curtailing habitat alteration, etc.) can be considered to ensure the long\u2010term sustainability of small, isolated populations.<\/jats:p>","DOI":"10.1111\/eva.70144","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T10:54:11Z","timestamp":1754996051000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genomic Analysis Reveals Inbreeding in an Island Population of Alexander Archipelago Wolves"],"prefix":"10.1111","volume":"18","author":[{"given":"Katherine E.","family":"Zarn","sequence":"first","affiliation":[{"name":"National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station U.S. Department of Agriculture, Forest Service  Missoula Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8534-3664","authenticated-orcid":false,"given":"Gretchen H.","family":"Roffler","sequence":"additional","affiliation":[{"name":"Division of Wildlife Conservation Alaska Department of Fish and Game  Juneau Alaska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7587-3004","authenticated-orcid":false,"given":"Marty","family":"Kardos","sequence":"additional","affiliation":[{"name":"Northwest Fisheries Science Center National Oceanic and Atmospheric Administration  Seattle Washington USA"},{"name":"Flathead Lake Biological Station University of Montana  Polson Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Good","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences University of Montana  Missoula Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Vanderpool","sequence":"additional","affiliation":[{"name":"National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station U.S. Department of Agriculture, Forest Service  Missoula Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor","family":"Wilcox","sequence":"additional","affiliation":[{"name":"National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station U.S. Department of Agriculture, Forest Service  Missoula Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3521-3367","authenticated-orcid":false,"given":"Michael K.","family":"Schwartz","sequence":"additional","affiliation":[{"name":"National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station U.S. Department of Agriculture, Forest Service  Missoula Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2011.0261"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.13797"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cobi.12109"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-246"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-04449-4"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/eth.13250"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009726"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.2.573"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-022-00448-x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.116.034678"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2664"},{"key":"e_1_2_9_14_1","first-page":"1","article-title":"An Evaluation of Potential Inbreeding Depression in Wild Mexican Wolves","volume":"88","author":"Clement M. 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