{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T05:49:44Z","timestamp":1791438584283,"version":"4.3.3"},"reference-count":57,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evolutionary Applications"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Accurate estimates of adult mosquito abundance are central to the design and evaluation of vector control strategies, yet they are difficult to obtain from natural populations. Conventional mark\u2010recapture methods for estimating adult mosquito census size pose logistical and other challenges. A recently developed close\u2010kin mark\u2010recapture (CKMR) approach is a promising alternative. However, application of CKMR has been largely confined to long\u2010lived vertebrates. Validation on empirical data from short\u2010lived, highly fecund insects is lacking. Here, we apply CKMR to a natural population of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Anopheles gambiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    , the primary African malaria mosquito, sampled from a small island in Lake Victoria, Uganda. Using a high\u2010diversity amplicon panel of genome\u2010wide markers, we genotyped 714 adult mosquitoes collected over a 20\u2010day period. We classified pairs to close\u2010kin categories by implementing probabilistic latent\u2010kinship estimation rather than using a deterministic threshold\u2010based framework. We observe numerous full\u2010sibling pairs but no parent\u2010offspring pairs, a pattern consistent with extreme variance in reproductive success owing to frequent failure of mosquito egg clutches to produce adults. Building on existing CKMR theory tailored to mosquito life history, we fit an extended model that included the possibility of clutch failure. By incorporating probabilities of both parent\u2010offspring and full\u2010sibling relationships, we estimate an adult female census size of 26,887 (95% credible interval: 6979\u2013146,011), and a clutch failure probability of 97.6% (CrI: 91.3%\u201399.6%). We use these estimates to calculate the predicted variance in reproductive success and effective population size. Through individual\u2010based simulations we confirm our estimates and the necessity of modeling reproductive variance to explain observed kinship patterns. Our results demonstrate that CKMR can be applied successfully to mosquitoes, provided that appropriate adjustments are made to account for their natural history.\n                  <\/jats:p>","DOI":"10.1111\/eva.70322","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:53:28Z","timestamp":1791435208000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Estimation of Adult Census Size From Close\u2010Kin Dyads in the Malaria Mosquito\n                    <i>Anopheles gambiae<\/i>"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3062-5901","authenticated-orcid":false,"given":"Anita","family":"Lerch","sequence":"first","affiliation":[{"name":"Department of Biological Sciences and Eck Institute for Global Health University of Notre Dame  Notre Dame Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott T.","family":"Small","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Eck Institute for Global Health University of Notre Dame  Notre Dame Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1702-803X","authenticated-orcid":false,"given":"Tin\u2010Yu J.","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Faculty of Natural Sciences Imperial College London, Silwood Park  Ascot UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3132-9776","authenticated-orcid":false,"given":"Priscila","family":"Bascu\u00f1\u00e1n","sequence":"additional","affiliation":[{"name":"CDC Foundation assigned to Centers for Disease Control and Prevention (CDC), Division of Parasitic Diseases and Malaria, Entomology Branch  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9236-9566","authenticated-orcid":false,"given":"Ellen M.","family":"Dotson","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), Division of Parasitic Diseases and Malaria Entomology Branch  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1447-3525","authenticated-orcid":false,"given":"Martin","family":"Lukindu","sequence":"additional","affiliation":[{"name":"Entomology Division Uganda Virus Research Institute (UVRI)  Entebbe Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krystal","family":"Birungi","sequence":"additional","affiliation":[{"name":"Entomology Division Uganda Virus Research Institute (UVRI)  Entebbe Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan K.","family":"Kayondo","sequence":"additional","affiliation":[{"name":"Entomology Division Uganda Virus Research Institute (UVRI)  Entebbe Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5146-9640","authenticated-orcid":false,"given":"Austin","family":"Burt","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Faculty of Natural Sciences Imperial College London, Silwood Park  Ascot UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7518-4014","authenticated-orcid":false,"given":"T. Alex","family":"Perkins","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Eck Institute for Global Health University of Notre Dame  Notre Dame Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0646-0721","authenticated-orcid":false,"given":"Nora J.","family":"Besansky","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Eck Institute for Global Health University of Notre Dame  Notre Dame Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"Anderson E. C.2022.\u201cCkmrsim: Inference of Pairwise Relationships Using Likelihood Ratios.\u201dR Package Version 0.1."},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1111\/2041-210X.14419","article-title":"Estimating Effective Population Size Using Close\u2010Kin Mark\u2013Recapture","volume":"15","author":"Babyn J.","year":"2024","journal-title":"Methods in Ecology and Evolution"},{"key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1111\/1755-0998.12737","article-title":"Microhaplotypes Provide Increased Power From Short\u2010Read DNA Sequences for Relationship Inference","volume":"18","author":"Baetscher D. S.","year":"2018","journal-title":"Molecular Ecology Resources"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1079\/BER2003259","article-title":"Effect of Temperature on the Development of the Aquatic Stages of \n                     Anopheles gambiae\n                   Sensu Stricto (Diptera: Culicidae)","volume":"93","author":"Bayoh M. N.","year":"2003","journal-title":"Bulletin of Entomological Research"},{"key":"e_1_2_11_6_1","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1111\/eva.12878","article-title":"Assessing Connectivity Despite High Diversity in Island Populations of a Malaria Mosquito","volume":"13","author":"Bergey C. M.","year":"2020","journal-title":"Evolutionary Applications"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1214\/16-STS552","article-title":"Close\u2010Kin Mark\u2010Recapture","volume":"31","author":"Bravington M. V.","year":"2016","journal-title":"Statistical Science"},{"key":"e_1_2_11_8_1","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1111\/1755-0998.12357","article-title":"Genotyping\u2010In\u2010Thousands by Sequencing (Gt\u2010Seq): A Cost Effective Snp Genotyping Method Based on Custom Amplicon Sequencing","volume":"15","author":"Campbell N. R.","year":"2015","journal-title":"Molecular Ecology Resources"},{"key":"e_1_2_11_9_1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1079\/BER2004297","article-title":"Key Premises, a Guide to \n                     Aedes aegypti\n                   (Diptera: Culicidae) Surveillance and Control","volume":"94","author":"Chadee D. D.","year":"2004","journal-title":"Bulletin of Entomological Research"},{"key":"e_1_2_11_10_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0011963","article-title":"Evaluation of Five Methods for Total DNA Extraction From Western Corn Rootworm Beetles","volume":"5","author":"Chen H.","year":"2010","journal-title":"PLoS One"},{"key":"e_1_2_11_11_1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1365-2915.1996.tb00733.x","article-title":"Density, Survival and Dispersal of \n                     Anopheles gambiae\n                   Complex Mosquitoes in a West African Sudan Savanna Village","volume":"10","author":"Costantini C.","year":"1996","journal-title":"Medical and Veterinary Entomology"},{"key":"e_1_2_11_12_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/gigascience\/giab008","article-title":"Twelve Years of Samtools and Bcftools","volume":"10","author":"Danecek P.","year":"2021","journal-title":"GigaScience"},{"key":"e_1_2_11_13_1","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/nature13987","article-title":"Signatures of Aestivation and Migration in Sahelian Malaria Mosquito Populations","volume":"516","author":"Dao A.","year":"2014","journal-title":"Nature"},{"key":"e_1_2_11_14_1","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1146\/annurev-ecolsys-031120-101013","article-title":"Gene Drive Dynamics in Natural Populations: The Importance of Density Dependence, Space, and Sex","volume":"51","author":"Dhole S.","year":"2020","journal-title":"Annual Review of Ecology, Evolution, and Systematics"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1186\/s13071-017-2310-6","article-title":"The Use of Sequential Mark\u2010Release\u2010Recapture Experiments to Estimate Population Size, Survival and Dispersal of Male Mosquitoes of the \n                     Anopheles gambiae\n                   Complex in Bana, a West African Humid Savannah Village","volume":"10","author":"Epopa P. S.","year":"2017","journal-title":"Parasites & Vectors"},{"key":"e_1_2_11_16_1","doi-asserted-by":"crossref","DOI":"10.1186\/s12915-020-00841-0","article-title":"Using Spatial Genetics to Quantify Mosquito Dispersal for Control Programs","volume":"18","author":"Filipovic I.","year":"2020","journal-title":"BMC Biology"},{"key":"e_1_2_11_17_1","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1186\/s12859-018-2579-2","article-title":"Ngmerge: Merging Paired\u2010End Reads via Novel Empirically\u2010Derived Models of Sequencing Errors","volume":"19","author":"Gaspar J. M.","year":"2018","journal-title":"BMC Bioinformatics"},{"key":"e_1_2_11_18_1","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1186\/1756-3305-7-276","article-title":"A Global Assembly of Adult Female Mosquito Mark\u2010Release\u2010Recapture Data to Inform the Control of Mosquito\u2010Borne Pathogens","volume":"7","author":"Guerra C. A.","year":"2014","journal-title":"Parasites & Vectors"},{"key":"e_1_2_11_19_1","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1093\/molbev\/msy228","article-title":"Slim 3: Forward Genetic Simulations Beyond the Wright\u2010Fisher Model","volume":"36","author":"Haller B. C.","year":"2019","journal-title":"Molecular Biology and Evolution"},{"key":"e_1_2_11_20_1","doi-asserted-by":"crossref","first-page":"8976","DOI":"10.1038\/s41467-024-53065-z","article-title":"The Potential of Gene Drives in Malaria Vector Species to Control Malaria in African Environments","volume":"15","author":"Hancock P. A.","year":"2024","journal-title":"Nature Communications"},{"key":"e_1_2_11_21_1","unstructured":"Hartig F. F.Minunno andS.Paul.2023.\u201cBayesiantools: General\u2010Purpose Mcmc and Smc Samplers and Tools for Bayesian Statistics R Package Version 0.1.8.\u201d"},{"key":"e_1_2_11_22_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1093\/biomet\/57.1.97","article-title":"Monte Carlo Sampling Methods Using Markov Chains and Their Application","volume":"57","author":"Hastings W.","year":"1970","journal-title":"Biometrika"},{"key":"e_1_2_11_23_1","doi-asserted-by":"crossref","first-page":"971","DOI":"10.5343\/bms.2010.1051","article-title":"Sweepstakes Reproductive Success in Highly Fecund Marine Fish and Shellfish: A Review and Commentary","volume":"87","author":"Hedgecock D.","year":"2011","journal-title":"Bulletin of Marine Science"},{"key":"e_1_2_11_24_1","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.1111\/1755-0998.13043","article-title":"A Genomic Approach to Inferring Kinship Reveals Limited Intergenerational Dispersal in the Yellow Fever Mosquito","volume":"19","author":"Jasper M.","year":"2019","journal-title":"Molecular Ecology Resources"},{"key":"e_1_2_11_25_1","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1111\/1755-0998.13520","article-title":"Estimating Dispersal Using Close Kin Dyads: The Kindisperse R Package","volume":"22","author":"Jasper M. E.","year":"2022","journal-title":"Molecular Ecology Resources"},{"key":"e_1_2_11_26_1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1475-2875-4-59","article-title":"Genetic Structure of \n                     Anopheles gambiae\n                   Populations on Islands in Northwestern Lake Victoria, Uganda","volume":"4","author":"Kayondo J. K.","year":"2005","journal-title":"Malaria Journal"},{"key":"e_1_2_11_27_1","volume-title":"Ecology: The Experimental Analysis of Distribution and Abundance","author":"Krebs C. J.","year":"2009"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1101\/2022.05.30.494059"},{"key":"e_1_2_11_29_1","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1603\/0022-2585-40.4.421","article-title":"Spatial and Temporal Variation in Kinship Among \n                     Anopheles gambiae\n                   (Diptera: Culicidae) Mosquitoes","volume":"40","author":"Lehmann T.","year":"2003","journal-title":"Journal of Medical Entomology"},{"key":"e_1_2_11_30_1","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","article-title":"Fast and Accurate Short Read Alignment With Burrows\u2010Wheeler Transform","volume":"25","author":"Li H.","year":"2009","journal-title":"Bioinformatics"},{"key":"e_1_2_11_31_1","doi-asserted-by":"crossref","first-page":"12512","DOI":"10.1038\/s41598-023-38639-z","article-title":"Close\u2010Kin Mark\u2010Recapture Informs Critically Endangered Terrestrial Mammal Status","volume":"13","author":"Lloyd\u2010Jones L. R.","year":"2023","journal-title":"Scientific Reports"},{"key":"e_1_2_11_32_1","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1186\/s13071-018-2826-4","article-title":"Spatio\u2010Temporal Genetic Structure of \n                     Anopheles gambiae\n                   in the Northwestern Lake Victoria Basin, Uganda: Implications for Genetic Control Trials in Malaria Endemic Regions","volume":"11","author":"Lukindu M.","year":"2018","journal-title":"Parasites & Vectors"},{"key":"e_1_2_11_33_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1013713","article-title":"Spatial Close\u2010Kin Mark\u2010Recapture Methods to Estimate Dispersal Parameters and Barrier Strength for Mosquitoes","volume":"21","author":"Marshall J. M.","year":"2025","journal-title":"PLoS Computational Biology"},{"key":"e_1_2_11_34_1","doi-asserted-by":"crossref","first-page":"18606","DOI":"10.1038\/s41598-022-20862-9","article-title":"Low Levels of Sibship Encourage Use of Larvae in Western Atlantic Bluefin Tuna Abundance Estimation by Close\u2010Kin Mark\u2010Recapture","volume":"12","author":"McDowell J. R.","year":"2022","journal-title":"Scientific Reports"},{"key":"e_1_2_11_35_1","doi-asserted-by":"crossref","DOI":"10.1002\/ece3.70230","article-title":"Assessing the Suitability of a One\u2010Time Sampling Event for Close\u2010Kin Mark\u2010Recapture: A Caribou Case Study","volume":"14","author":"Merriell B. D.","year":"2024","journal-title":"Ecology and Evolution"},{"key":"e_1_2_11_36_1","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1063\/1.1699114","article-title":"Equations of State Calculations by Fast Computing Machines","volume":"21","author":"Metropolis N.","year":"1953","journal-title":"Journal of Chemical Physics"},{"key":"e_1_2_11_37_1","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/nature24995","article-title":"Genetic Diversity of the African Malaria Vector \n                     Anopheles gambiae","volume":"552","author":"Miles A.","year":"2017","journal-title":"Nature"},{"key":"e_1_2_11_38_1","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adj6990","article-title":"Predicting the Age of Field Anopheles Mosquitoes Using Mass Spectrometry and Deep Learning","volume":"10","author":"Mohammad N.","year":"2024","journal-title":"Science Advances"},{"key":"e_1_2_11_39_1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/s12936-019-2708-z","article-title":"Artisanal Fishing Supports Breeding of Malaria Mosquitoes in Western Kenya","volume":"18","author":"Mukabana W. R.","year":"2019","journal-title":"Malaria Journal"},{"key":"e_1_2_11_40_1","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1093\/jmedent\/44.5.758","article-title":"Survivorship of Immature Stages of \n                     Anopheles gambiae\n                   S.L. (Diptera: Culicidae) in Natural Habitats in Western Kenya Highlands","volume":"44","author":"Munga S.","year":"2007","journal-title":"Journal of Medical Entomology"},{"key":"e_1_2_11_41_1","doi-asserted-by":"crossref","first-page":"54","DOI":"10.4269\/ajtmh.2006.74.54","article-title":"Pupal Habitat Productivity of \n                     Anopheles gambiae\n                   Complex Mosquitoes in a Rural Village in Western Kenya","volume":"74","author":"Mutuku F. M.","year":"2006","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"e_1_2_11_42_1","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/s12936-025-05768-x","article-title":"Assessing the Population Genetic Structure and Demographic History of Anopheles Gambiae and Anopheles Arabiensis at Island and Mainland Sites in Uganda: Implications for Testing Novel Malaria Vector Control Approaches","volume":"25","author":"Mwima R.","year":"2026","journal-title":"Malaria Journal"},{"key":"e_1_2_11_43_1","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.1111\/evo.13063","article-title":"Effective Size of Density\u2010Dependent Populations in Fluctuating Environments","volume":"70","author":"Myhre A. M.","year":"2016","journal-title":"Evolution"},{"key":"e_1_2_11_44_1","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1002\/ece3.329","article-title":"Effective\/Census Population Size Ratio Estimation: A Compendium and Appraisal","volume":"2","author":"Palstra F. P.","year":"2012","journal-title":"Ecology and Evolution"},{"key":"e_1_2_11_45_1","doi-asserted-by":"crossref","DOI":"10.1111\/1755-0998.70074","article-title":"Simulation\u2010Based Spatially Explicit Close\u2010Kin Mark\u2010Recapture","volume":"26","author":"Patterson G.","year":"2026","journal-title":"Molecular Ecology Resources"},{"key":"e_1_2_11_46_1","first-page":"7","article-title":"Coda: Convergence Diagnosis and Output Analysis for Mcmc","volume":"6","author":"Plummer M.","year":"2006","journal-title":"R News"},{"key":"e_1_2_11_47_1","doi-asserted-by":"crossref","DOI":"10.3389\/fgene.2021.780327","article-title":"Monitoring Needs for Gene Drive Mosquito Projects: Lessons From Vector Control Field Trials and Invasive Species","volume":"12","author":"Rasic G.","year":"2021","journal-title":"Frontiers in Genetics"},{"key":"e_1_2_11_48_1","doi-asserted-by":"crossref","DOI":"10.1186\/1475-2875-7-163","article-title":"Insertion Polymorphisms of Sine200 Retrotransposons Within Speciation Islands of \n                     Anopheles gambiae\n                   Molecular Forms","volume":"7","author":"Santolamazza F.","year":"2008","journal-title":"Malaria Journal"},{"key":"e_1_2_11_49_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1086\/722175","article-title":"Close Kin Dyads Indicate Intergenerational Dispersal and Barriers","volume":"201","author":"Schmidt T. L.","year":"2023","journal-title":"American Naturalist"},{"key":"e_1_2_11_50_1","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1111\/mec.15792","article-title":"Spatial Population Genomics of a Recent Mosquito Invasion","volume":"30","author":"Schmidt T. L.","year":"2021","journal-title":"Molecular Ecology"},{"key":"e_1_2_11_51_1","volume-title":"The Estimation of Animal Abundance and Related Parameters","author":"Seber G. A. F.","year":"1982"},{"key":"e_1_2_11_52_1","first-page":"169","article-title":"Studies on Sampling Larval Populations of the \n                     Anopheles gambiae\n                   Complex","volume":"45","author":"Service, M. W","year":"1971","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_11_53_1","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1017\/S0007485300003862","article-title":"Mortalities of the Larvae of the \n                     Anopheles gambiae\n                   Giles Complex and Detection of Predators by the Precipitin Test","volume":"62","author":"Service, M. W","year":"1973","journal-title":"Bulletin of Entomological Research"},{"key":"e_1_2_11_54_1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1093\/jmedent\/13.4-5.535","article-title":"Mortalities of the Immature Stages of Species B of the \n                     Anopheles gambiae\n                   Complex in Kenya: Comparison Between Rice Fields and Temporary Pools, Identification of Predators, and Effects of Insecticidal Spraying","volume":"13","author":"Service, M. W","year":"1977","journal-title":"Journal of Medical Entomology"},{"key":"e_1_2_11_55_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1010755","article-title":"Close\u2010Kin Mark\u2010Recapture Methods to Estimate Demographic Parameters of Mosquitoes","volume":"18","author":"Sharma Y.","year":"2022","journal-title":"PLoS Computational Biology"},{"key":"e_1_2_11_56_1","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1603\/033.046.0602","article-title":"A New, Cost\u2010Effective, Battery\u2010Powered Aspirator for Adult Mosquito Collections","volume":"46","author":"Vazquez\u2010Prokopec G. M.","year":"2009","journal-title":"Journal of Medical Entomology"},{"key":"e_1_2_11_57_1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1186\/s12936-018-2432-0","article-title":"Reduced\u2010Representation Sequencing Identifies Small Effective Population Sizes of \n                     Anopheles gambiae\n                   in the North\u2010Western Lake Victoria Basin, Uganda","volume":"17","author":"Wiltshire R. M.","year":"2018","journal-title":"Malaria Journal"},{"key":"e_1_2_11_58_1","doi-asserted-by":"crossref","DOI":"10.30875\/9789287076717","volume-title":"World Malaria Report 2024: Addressing Inequity in the Global Malaria Response","author":"World Health Organization","year":"2024"}],"container-title":["Evolutionary Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eva.70322","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/eva.70322","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eva.70322","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:53:55Z","timestamp":1791435235000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/eva.70322"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":57,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/eva.70322"],"URL":"https:\/\/doi.org\/10.1111\/eva.70322","archive":["Portico"],"relation":{},"ISSN":["1752-4571","1752-4571"],"issn-type":[{"value":"1752-4571","type":"print"},{"value":"1752-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70322"}}