{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T10:59:27Z","timestamp":1790593167283,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005621","name":"Kasetsart University Research and Development Institute","doi-asserted-by":"publisher","award":["FF(KU) 61.69"],"award-info":[{"award-number":["FF(KU) 61.69"]}],"id":[{"id":"10.13039\/501100005621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011289","name":"International Society of Biomechanics","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011289","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evolutionary Applications"],"published-print":{"date-parts":[[2026,9]]},"abstract":"ABSTRACT<\/jats:title>\n \n The production of clariid catfish in Thai aquaculture depends on culture of the native species (\n \n Clarias macrocephalus<\/jats:italic>\n <\/jats:styled-content>\n and\n \n C.\u2009batrachus<\/jats:italic>\n <\/jats:styled-content>\n ) and introduced African catfish (\n \n C.\u2009gariepinus<\/jats:italic>\n <\/jats:styled-content>\n ). Particularly, hybridization with\n \n C.\u2009gariepinus<\/jats:italic>\n <\/jats:styled-content>\n has improved productivity but raised significant conservation concerns. These lineages show distinct growth variations; however, the underlying genetic factors remain uncharacterized. We performed a comprehensive genomic assessment of the myostatin\u2010b (\n Mstnb<\/jats:italic>\n ) gene in 535 individuals comprising 19 Thai populations. The study design involved a novel integration of high\u2010throughput sequencing, structural modeling, and multi\u2010locus analysis. We identified eight novel alleles defined by 19 variable sites within exon 1. The observed diversity challenges the paradigm of extreme sequence conservation that typically occurs in vertebrate Myostatin. Although Bayesian phylogenetics showed intermingled allelic lineages, an integrated \u201cgrowth\u2010gene pool\u201d approach that combined\n Mstnb<\/jats:italic>\n and\n GH1<\/jats:italic>\n polymorphisms resolved a robust species\u2010level split (\n K<\/jats:italic>\n \u2009=\u20092) and hierarchical sub\u2010structuring (\n K<\/jats:italic>\n \u2009=\u20096). Selection analyses identified significant adaptive diversification (\n \u03c9<\/jats:italic>\n \u2009=\u20091.857). Codon\u2010level models highlighted Site 95 as a primary target of pervasive diversifying selection. Moreover, a nonsense mutation that resulted in a premature stop codon was identified as the mechanism underlying the functional modulation of growth traits. These findings provide a high\u2010resolution molecular toolkit for marker\u2010assisted selection and invasive species monitoring, which would enhance the sustainability of clariid aquaculture and protect native biodiversity against the risks of anthropogenic hybridization.\n <\/jats:p>","DOI":"10.1111\/eva.70324","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:37:13Z","timestamp":1790224633000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adaptive Diversification of the\n Mstnb<\/i>\n Locus Drives Genomic Architecture of Growth in Thai Clariid Catfish"],"prefix":"10.1111","volume":"19","author":[{"given":"Ton Huu Duc","family":"Nguyen","sequence":"first","affiliation":[{"name":"Animal Genomics and Bioresource Research Unit (AGB Research Unit), Faculty of Science Kasetsart University Bangkok Thailand"},{"name":"Interdisciplinary Graduate Program in Bioscience, Faculty of Science Kasetsart University Bangkok Thailand"},{"name":"School of Education, Faculty of Biology Education Can Tho University Can Tho Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phonemany","family":"Thammachak","sequence":"additional","affiliation":[{"name":"Animal Genomics and Bioresource Research Unit (AGB Research Unit), Faculty of Science Kasetsart University Bangkok Thailand"},{"name":"Department of Genetics, Faculty of Science Kasetsart University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7589-3227","authenticated-orcid":false,"given":"Trifan","family":"Budi","sequence":"additional","affiliation":[{"name":"Animal Genomics and Bioresource Research Unit (AGB Research Unit), Faculty of Science Kasetsart University Bangkok Thailand"},{"name":"School of Agricultural Technology King Mongkut's Institute of Technology Ladkrabang Bangkok 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