{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:44:07Z","timestamp":1792010647136,"version":"4.4.0"},"reference-count":173,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evolutionary Applications"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sustainable fisheries rely on balancing harvests with ecological impacts. Despite the importance of species' demographic profiles, many assessments forgo age due to methodological complexities. Developing non\u2010lethal methods for age determination can improve stock assessments to ensure future sustainability. Epigenetic signals, specifically DNA methylation, are dynamic and considered to be associated with decreasing genomic stability with age. Subsequently, the modelling of methylation percentages at CpG loci has been utilized to accurately predict chronological age in many organisms, including several fish species. We examined the influence of age (1\u201314\u2009years), location (Texas versus Tennessee), and sex on DNA methylation patterns of members of the largemouth bass\u2010complex (LBc), consisting of largemouth bass (\n                    <jats:italic>Micropterus nigricans<\/jats:italic>\n                    ), Florida bass (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Micropterus salmoides<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), and their resulting hybrids, all recreational and commercially important species. As DNA methylation patterns are influenced by sex and environmental signals, we accounted for this when evaluating aging patterns in the LBc. Our results indicate global methylation patterns decrease with age, indicating potential genomic instability, and vary with sex and location. Non\u2010random changes also occur involving physiological pathways of diverse function. Accounting for this variability, aging clocks using single or regions of CpG loci were derived from 124 LBc samples and validated using sets of na\u00efve (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200932) individuals, providing improved estimates over other non\u2010lethal aging methods. Our results reveal insights into dynamic methylome patterns in the LBc, and when accounting for variability, informative and accurate age estimations can be determined with non\u2010lethal molecular methods.\n                  <\/jats:p>","DOI":"10.1111\/eva.70328","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:45:11Z","timestamp":1792007111000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Epigenetic Clocks Can Predict Age in the Largemouth Bass\u2010Complex Despite Spatial and Sexual Variation"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3997-2643","authenticated-orcid":false,"given":"Steven T.","family":"Gardner","sequence":"first","affiliation":[{"name":"Environmental Sciences Division Oak Ridge National Laboratory  Oak Ridge Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J.","family":"Daugherty","sequence":"additional","affiliation":[{"name":"Heart of the Hills Fisheries Science Center Texas Parks and Wildlife Department  Mountain Home Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dijar","family":"Lutz\u2010Carrillo","sequence":"additional","affiliation":[{"name":"Analytical Services Laboratory Texas Parks and Wildlife Department  San Marcos Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9073-6254","authenticated-orcid":false,"given":"Brenda","family":"Pracheil","sequence":"additional","affiliation":[{"name":"Earth Systems Science Division Pacific Northwest National Laboratory  Richland Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5697-2736","authenticated-orcid":false,"given":"Kristine N.","family":"Moody","sequence":"additional","affiliation":[{"name":"Environmental Sciences Division Oak Ridge National Laboratory  Oak Ridge Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2023.739350"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2012-13-10-r87"},{"key":"e_1_2_9_4_1","volume-title":"Genetics, Epigenetic Mechanism","author":"Al Aboud N. 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