{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:00:08Z","timestamp":1790110808343,"version":"4.0.1"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T00:00:00Z","timestamp":1365465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2013,11]]},"abstract":"Summary<\/jats:title>\n \n Reasons for performing study<\/jats:title>\n Intralesional (i.l.) injection is currently the most commonly used technique for stem cell therapy in equine tendon injury. A comparison of different techniques of injection of mesenchymal stem cells for the treatment of tendon lesions is required.<\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n We hypothesised that vascular perfusion of the equine distal limb with mesenchymal stem cells (\n MSC<\/jats:styled-content>\n s) would result in preferential distribution of\n MSC<\/jats:styled-content>\n s to acute tendon injuries.\n <\/jats:p>\n <\/jats:sec>\n \n Study design<\/jats:title>\n \n In vivo<\/jats:italic>\n experimental study.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Lesions were surgically induced in forelimb superficial digital flexor tendons of 8 horses. Three or 10 days after lesion induction, technetium\u201099 hexamethyl propylene amine oxime\u2010labelled\n MSC<\/jats:styled-content>\n s were injected via i.v. or intra\u2010arterial (i.a.) regional limb perfusion (\n RLP<\/jats:styled-content>\n ) at the level of the distal antebrachium and compared to i.l. injection. Mesenchymal stem cell persistence and distribution within the forelimb and tendon lesions was assessed with scintigraphy for 24\u2009h.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Lesion uptake was higher with i.l. injection than with\n RLP<\/jats:styled-content>\n , but\n MSC<\/jats:styled-content>\n persistence decreased similarly over time in all 3 techniques. Intra\u2010arterial\n RLP<\/jats:styled-content>\n resulted in a better distribution of\n MSC<\/jats:styled-content>\n s and a higher uptake at the lesion site than i.v.\n RLP<\/jats:styled-content>\n . Limbs perfused i.a. on Day 10 showed greater accumulation of\n MSC<\/jats:styled-content>\n s in the lesion than limbs perfused on Day 3. Arterial thrombosis occurred in 50% of the i.v.\n RLP<\/jats:styled-content>\n limbs and in 100% of the i.a.\n RLP<\/jats:styled-content>\n limbs, which led to clinical complications in one horse.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions and potential relevance<\/jats:title>\n \n Compared with i.l. injection,\n RLP<\/jats:styled-content>\n results in lower uptake but similar persistence of\n MSC<\/jats:styled-content>\n s at the site of tendon lesions. A time dependent accumulation of\n MSC<\/jats:styled-content>\n s was identified with i.a.\n RLP<\/jats:styled-content>\n . The i.a.\n RLP<\/jats:styled-content>\n appears more advantageous than the i.v.\n RLP<\/jats:styled-content>\n in terms of distribution and uptake. However, the described i.a. technique produced arterial thrombosis and thus cannot currently be recommended for clinical use.\n <\/jats:p>\n \n \n The\n S<\/jats:styled-content>\n ummary is available in\n C<\/jats:styled-content>\n hinese \u2013 see\n S<\/jats:styled-content>\n upporting information.\n <\/jats:bold>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/evj.12063","type":"journal-article","created":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T18:55:01Z","timestamp":1361213701000},"page":"726-731","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Distribution and persistence of technetium\u201099 hexamethyl propylene amine oxime\u2010labelled bone marrow\u2010derived mesenchymal stem cells in experimentally induced tendon lesions after intratendinous injection and regional perfusion of the equine distal limb"],"prefix":"10.1111","volume":"45","author":[{"given":"A.","family":"Sole","sequence":"first","affiliation":[{"name":"Veterinary Medical Teaching Hospital School of Veterinary Medicine University of California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Spriet","sequence":"additional","affiliation":[{"name":"Department of Surgical and Radiological Sciences School of Veterinary Medicine University of California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. A.","family":"Padgett","sequence":"additional","affiliation":[{"name":"Department of Surgical and Radiological Sciences School of Veterinary Medicine University of California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Vaughan","sequence":"additional","affiliation":[{"name":"Department of Surgical and Radiological Sciences School of Veterinary Medicine University of California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. D.","family":"Galuppo","sequence":"additional","affiliation":[{"name":"Department of Surgical and Radiological Sciences School of Veterinary Medicine University of California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. L.","family":"Borjesson","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology School of Veterinary Medicine University of California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. R.","family":"Wisner","sequence":"additional","affiliation":[{"name":"Department of Surgical and Radiological Sciences School of Veterinary Medicine University of California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Vidal","sequence":"additional","affiliation":[{"name":"Department of Surgical and Radiological Sciences School of Veterinary Medicine University of California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516409X477263"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/09638280701788241"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2011.00363.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.73.9.1435"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2011.00530.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22091"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22701"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.2741\/3853"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(18)31954-4"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3415\/VCOT-09-10-0106"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0838.2010.01164.x"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.71.10.1237"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/904682"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2008.0224"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.269"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-8261.2009.01536.x"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2010.00112.x"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20887"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-006-0245-0"}],"container-title":["Equine Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fevj.12063","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/evj.12063","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T13:50:21Z","timestamp":1762091421000},"score":1,"resource":{"primary":{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/10.1111\/evj.12063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,9]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1111\/evj.12063"],"URL":"https:\/\/doi.org\/10.1111\/evj.12063","archive":["Portico"],"relation":{},"ISSN":["0425-1644","2042-3306"],"issn-type":[{"value":"0425-1644","type":"print"},{"value":"2042-3306","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,9]]},"assertion":[{"value":"2012-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-01-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}