{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:06:17Z","timestamp":1770221177524,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,8,30]],"date-time":"2013-08-30T00:00:00Z","timestamp":1377820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2014,1]]},"abstract":"Summary<\/jats:title>\n \n Reasons for performing study<\/jats:title>\n \n There is little published information on whether measurement of plasma\n ACTH<\/jats:styled-content>\n concentration at a single timepoint is a repeatable indicator of pituitary\n pars intermedia<\/jats:italic>\n dysfunction (\n PPID<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n To determine whether ultradian or circadian fluctuations in\n ACTH<\/jats:styled-content>\n production influence plasma\n ACTH<\/jats:styled-content>\n concentration in normal horses and horses with\n PPID<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Study design<\/jats:title>\n Prospective observational study.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Plasma\n ACTH<\/jats:styled-content>\n concentration in 8 non\u2010\n PPID<\/jats:styled-content>\n horses and 8 horses with\n PPID<\/jats:styled-content>\n was measured at 08.00, 11.00, 14.00 and 17.00\u2009h on 5 nonconsecutive days within a 3 week period. In addition, at 08.30\u2009h on one day, 6 samples were collected from each horse at precisely 5\u2009min intervals over a period of 25\u2009min. Descriptive and graphical analysis was performed and a linear mixed effects model was fitted to assess the effect of time of day on\n ACTH<\/jats:styled-content>\n concentration in non\u2010\n PPID<\/jats:styled-content>\n and\n PPID<\/jats:styled-content>\n horses.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Evidence of ultradian fluctuation in\n ACTH<\/jats:styled-content>\n production was not identified in either non\u2010\n PPID<\/jats:styled-content>\n or\n PPID<\/jats:styled-content>\n horses. Evidence for circadian fluctuation was identified in non\u2010\n PPID<\/jats:styled-content>\n horses; plasma\n ACTH<\/jats:styled-content>\n concentrations were highest at 08.00\u2009h and decreased through the day. There was no evidence of circadian fluctuation in\n PPID<\/jats:styled-content>\n horses. In non\u2010\n PPID<\/jats:styled-content>\n horses, the magnitude of circadian changes in\n ACTH<\/jats:styled-content>\n concentration was smaller than variations in concentration that occurred at random. Intrahorse variability of\n ACTH<\/jats:styled-content>\n concentration was greater in\n PPID<\/jats:styled-content>\n horses than in non\u2010\n PPID<\/jats:styled-content>\n horses.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Ultradian and circadian fluctuations in\n ACTH<\/jats:styled-content>\n concentration are unlikely to influence clinical decision making; however, variations of potential clinical relevance do occur in individual horses, for reasons that remain to be determined, and increase in magnitude with progression of\n PPID<\/jats:styled-content>\n . Results of the current study indicate that when an\n ACTH<\/jats:styled-content>\n concentration between 19 and 40\u2009pg\/ml is measured, further testing should be considered to increase the accuracy of\n PPID<\/jats:styled-content>\n diagnosis.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/evj.12114","type":"journal-article","created":{"date-parts":[[2013,6,7]],"date-time":"2013-06-07T03:59:44Z","timestamp":1370577584000},"page":"113-117","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Investigation of rhythms of secretion and repeatability of plasma adrenocorticotropic hormone concentrations in healthy horses and horses with pituitary\n pars intermedia<\/i>\n dysfunction"],"prefix":"10.1111","volume":"46","author":[{"given":"D. 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