{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T03:14:25Z","timestamp":1785122065005,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,9,17]],"date-time":"2013-09-17T00:00:00Z","timestamp":1379376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2014,5]]},"abstract":"Summary<\/jats:title>\n \n Reasons for performing study<\/jats:title>\n \n Red complex bacteria, i.e.\n \n P<\/jats:styled-content>\n orphyromonas gingivalis\n <\/jats:italic>\n ,\n \n T<\/jats:styled-content>\n reponema denticola\n <\/jats:italic>\n and\n \n T<\/jats:styled-content>\n annerella forsythia,\n <\/jats:italic>\n are involved in the onset and progression of periodontal disease in man, yet seldom inhabit the oral cavity of healthy individuals. Periodontal disease is also encountered in horses, with equine odontoclastic tooth resorption and hypercementosis (\n EOTRH<\/jats:styled-content>\n ) constituting a particular form of disease. However, only little is known about the oral microbiome of healthy and periodontitis\u2010affected equids.\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n We aimed to test the hypothesis that red complex bacteria are also associated with\n EOTRH<\/jats:styled-content>\n \u2010related periodontal disease.\n <\/jats:p>\n <\/jats:sec>\n \n Study design<\/jats:title>\n Controlled cross\u2010sectional study.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We screened\n DNA<\/jats:styled-content>\n purified from crevicular fluid derived from 23\n EOTRH<\/jats:styled-content>\n \u2010affected and 21 disease\u2010free horses for the presence of\n \n T<\/jats:styled-content>\n reponema\n <\/jats:italic>\n spp.,\n \n T<\/jats:styled-content>\n annerella\n <\/jats:italic>\n spp. and\n \n P<\/jats:styled-content>\n orphyromonas gingivalis\n <\/jats:italic>\n \u2005\n DNA<\/jats:styled-content>\n by polymerase chain reaction. Subsequently, amplified\n DNA<\/jats:styled-content>\n was bidirectionally sequenced and identified via\n BLAST<\/jats:styled-content>\n analysis.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n \n T<\/jats:styled-content>\n reponema\n <\/jats:italic>\n and\/or\n \n T<\/jats:styled-content>\n annerella\n <\/jats:italic>\n \u2005\n DNA<\/jats:styled-content>\n was detected in 100% of periodontitis\u2010related samples and in 52.2% of\n DNA<\/jats:styled-content>\n derived from healthy horses. Twenty\u2010six amplicon sequences were 98\u2013100% homologous to published bacterial sequences, which mostly corresponded to\n \n T<\/jats:styled-content>\n reponema pectinovorum\n <\/jats:italic>\n , oral\n \n T<\/jats:styled-content>\n reponema\n <\/jats:italic>\n clones\n JU025<\/jats:styled-content>\n and\n OMZ<\/jats:styled-content>\n 840, and\n \n T<\/jats:styled-content>\n annerella forsythia\n <\/jats:italic>\n .\n \n P<\/jats:styled-content>\n .\u2009gingivalis\n <\/jats:italic>\n \u2005\n DNA<\/jats:styled-content>\n was only found in 3\n EOTRH<\/jats:styled-content>\n \u2010related samples. Forty\u2010three amplicon sequences revealed weaker homologies ranging between 80% and 97% to known\n \n T<\/jats:styled-content>\n reponema\n <\/jats:italic>\n or\n \n T<\/jats:styled-content>\n annerella\n <\/jats:italic>\n strains, partly because of their heterogeneity, partly because they obviously represented so far unknown types.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n This is the first report in which known and novel\n \n T<\/jats:styled-content>\n reponema\n <\/jats:italic>\n and\n \n T<\/jats:styled-content>\n annerella\n <\/jats:italic>\n spp. were isolated in association with\n EOTRH<\/jats:styled-content>\n \u2010related periodontal disease.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/evj.12115","type":"journal-article","created":{"date-parts":[[2013,6,7]],"date-time":"2013-06-07T03:59:45Z","timestamp":1370577585000},"page":"358-363","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Isolation of\n \n T<\/scp>\n reponema\n <\/i>\n and\n \n T<\/scp>\n annerella\n <\/i>\n spp. from equine odontoclastic tooth resorption and hypercementosis related periodontal disease"],"prefix":"10.1111","volume":"46","author":[{"given":"S.","family":"Sykora","sequence":"first","affiliation":[{"name":"Research Group Oncology (RGO) Equine Clinic University of Veterinary Medicine Austria"},{"name":"Clinic of Large Animal Surgery Equine Clinic University of Veterinary Medicine Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Pieber","sequence":"additional","affiliation":[{"name":"Clinic of Large Animal Surgery Equine Clinic University of Veterinary Medicine Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Simhofer","sequence":"additional","affiliation":[{"name":"Clinic of Large Animal Surgery Equine Clinic University of Veterinary Medicine Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Hackl","sequence":"additional","affiliation":[{"name":"Research Group Oncology (RGO) Equine Clinic University of Veterinary Medicine Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Brodesser","sequence":"additional","affiliation":[{"name":"Research Group Oncology (RGO) Equine Clinic University of Veterinary Medicine Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Brandt","sequence":"additional","affiliation":[{"name":"Research Group Oncology (RGO) Equine Clinic University of Veterinary Medicine Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,9,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01706.x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2010.08.002"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ctep.2005.04.005"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2002.280102.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2002.290313.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-03010-9"},{"key":"e_1_2_11_9_1","unstructured":"Baker G.J.(1979)A Study of Dental Disease in the Horse PhD Thesis. Faculty of Veterinary Medicine University of Glasgow UK."},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(91)90030-J"},{"key":"e_1_2_11_11_1","unstructured":"Bienert A.(2002)Digitalradiographische computertomographische und mikrobiologische Untersuchungen bei Backenzahnerkrankungen des Pferdes PhD Thesis. Tier\u00e4rztliche Hochschule Hannover Germany."},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1987.tb01435.x"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.04.026"},{"key":"e_1_2_11_14_1","unstructured":"Baratt R.M.(2007)Equine incisor resorptive lesions. In:Proceedings of the 21st Annual Veterinary Dental Forum October 21\u201024 2007 Minneapolis Minnesota; pp23\u201030."},{"key":"e_1_2_11_15_1","unstructured":"Caldwell L.A.(2007)Clinical features of chronic disease of the anterior dentition in horses. In:Proceedings of the 21st Annual Veterinary Dental Forum October 21\u201024 Minneapolis Minnesota USA pp18\u201021."},{"key":"e_1_2_11_16_1","first-page":"N23","article-title":"Equine odontoclastic tooth resorption and hypercementosis","volume":"88","author":"Lee S.","year":"2010","journal-title":"Aust. Vet. J."},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2008.09.017"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.44-4-533"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83568-0"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.08.031"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.09-0404"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.02.014"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00799.x"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.8.1406"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00199.x"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2008.01298.x"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-5037.2006.00168.x"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00113.x"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1994.tb00020.x"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02454.x"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02446.x"},{"key":"e_1_2_11_32_1","first-page":"9","volume-title":"Pathogenic Treponema \u2010 Molecular and Cellular Biology","author":"Paster B.J.","year":"2006"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1177\/089875649601300401"},{"key":"e_1_2_11_34_1","first-page":"23","article-title":"Isolation of oral spirochetes from dogs and cats and provisional identification using polymerase chain reaction (PCR) analysis specific for human plaque Treponema spp","volume":"17","author":"Valdez M.","year":"2000","journal-title":"J. Vet. Dent."},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.09.011"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.23.6860-6869.2003"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.10.016"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3920\/BM2010.0009"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-4388.84681"},{"key":"e_1_2_11_40_1","first-page":"95\u20101\u20102","article-title":"Creation of the gingival immunologic defense index (GIDI) to evaluate the immunological potential of the gingiva and the possible risk for periodontal disease","volume":"6","author":"De Souza\u2010Gugelmin M.C.","year":"1995","journal-title":"Braz. Dent. J."},{"key":"e_1_2_11_41_1","first-page":"105","article-title":"Changes in saliva protein composition in patients with periodontal disease","volume":"22","author":"Koss M.A.","year":"2009","journal-title":"Acta Odontol. Latinoam."},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5271.70"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-3-1179"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.12.018"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2011.06.002"}],"container-title":["Equine Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fevj.12115","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/evj.12115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T08:31:26Z","timestamp":1762072286000},"score":1,"resource":{"primary":{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/10.1111\/evj.12115"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,17]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["10.1111\/evj.12115"],"URL":"https:\/\/doi.org\/10.1111\/evj.12115","archive":["Portico"],"relation":{},"ISSN":["0425-1644","2042-3306"],"issn-type":[{"value":"0425-1644","type":"print"},{"value":"2042-3306","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,17]]},"assertion":[{"value":"2012-12-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-05-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}