{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:10:13Z","timestamp":1789387813875,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,1,3]],"date-time":"2014-01-03T00:00:00Z","timestamp":1388707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Morris Animal Foundation (MAF)","award":["D10EQ-805A"],"award-info":[{"award-number":["D10EQ-805A"]}]},{"name":"Morris Animal Foundation (MAF)","award":["D13EQ-602"],"award-info":[{"award-number":["D13EQ-602"]}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2014,11]]},"abstract":"Summary<\/jats:title>\n \n Reason for performing study<\/jats:title>\n \n Intraoperative assessment of colonic viability can be challenging and largely subjective. Objective methods are often impractical. Viability is related to re\u2010establishment of colonic perfusion; particularly microvascular perfusion. This study evaluated the utility of dark\u2010field microscopy (\n DFM<\/jats:styled-content>\n ) of the colonic serosa as an objective method of assessing microperfusion.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n To measure microvascular perfusion indices (\n MPI<\/jats:styled-content>\n ) of the pelvic flexure serosa in horses with surgical colonic lesions and correlate these with macroperfusion indices (\n MaPI<\/jats:styled-content>\n ) and histomorphometry.\n <\/jats:p>\n <\/jats:sec>\n \n Study design<\/jats:title>\n Prospective, clinical, case\u2013control study.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Control horses and horses with colonic volvulus (\n LCV<\/jats:styled-content>\n ), displacement, and\/or simple obstruction undergoing surgery had\n DFM<\/jats:styled-content>\n video loops performed on the pelvic flexure. Total vessel density, perfused vessel density, proportion of perfusion vessels and microvascular flow index were calculated from video analysis. Macroperfusion indices (arterial blood pressure and heart rate) were recorded. Histomorphometry was used to determine a mucosal injury score. Differences between lesions for\n MPI<\/jats:styled-content>\n ,\n MaPI<\/jats:styled-content>\n and histomorphometry were compared using\n ANOVA<\/jats:styled-content>\n or\n K<\/jats:styled-content>\n ruskal\u2013\n W<\/jats:styled-content>\n allis statistic. Spearman correlations between\n MPI<\/jats:styled-content>\n with\n MaPI<\/jats:styled-content>\n were performed. Linear regression was used to assess the relationship between\n MPI<\/jats:styled-content>\n and histomorphometry. P<0.05 was significant.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Horses with\n LCV<\/jats:styled-content>\n had lower perfused vessel density, proportion of microvascular perfusion vessels and flow index than horses with nonstrangulating obstructions and control horses. Macroperfusion indices were not correlated with\n MPI<\/jats:styled-content>\n but\n MPI<\/jats:styled-content>\n were correlated with histomorphometry.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Dark\u2010field microscopy is achievable in the operating room and can quantify\n MPI<\/jats:styled-content>\n from the colonic serosa in different colonic lesions. Macroperfusion indices were not related with colonic\n MPI<\/jats:styled-content>\n . Microvascular perfusion indices can predict the severity of histopathological change at the pelvic flexure. Derangements of\n MPI<\/jats:styled-content>\n may be more useful indicators of colonic pathology and viability and offer a more objective assessment of intestinal injury than subjective methods. Further study is needed to determine the utility of\n DFM<\/jats:styled-content>\n in predicting survival in horses with\n LCV<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/evj.12202","type":"journal-article","created":{"date-parts":[[2013,10,25]],"date-time":"2013-10-25T02:42:03Z","timestamp":1382668923000},"page":"674-680","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Dark\u2010field microscopy in the assessment of large colon microperfusion and mucosal injury in naturally occurring surgical disease of the equine large colon"],"prefix":"10.1111","volume":"46","author":[{"given":"S. D.","family":"Hurcombe","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine The Ohio State University USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. R.","family":"Welch","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine The Ohio State University USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine The Ohio State University USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. S.","family":"Cooper","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine The Ohio State University USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences College of Veterinary Medicine The Ohio State University USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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