{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T03:35:23Z","timestamp":1777779323745,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,10,7]],"date-time":"2014-10-07T00:00:00Z","timestamp":1412640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"School of Animal and Veterinary Sciences, Charles Sturt University"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2015,9]]},"abstract":"Summary<\/jats:title>\n \n Reasons for performing study<\/jats:title>\n \n Previous reports suggest that adrenocorticotrophic hormone (\n ACTH<\/jats:styled-content>\n ) degrades rapidly, limiting its use as a diagnostic test.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n This study quantified effects of processing delays on\n ACTH<\/jats:styled-content>\n concentrations and investigated the addition of\n \n N<\/jats:styled-content>\n <\/jats:italic>\n \u2010phenylmaleimide (maleimide), a protease inhibitor, as a means of reducing\n ACTH<\/jats:styled-content>\n degradation.\n <\/jats:p>\n <\/jats:sec>\n \n Study design<\/jats:title>\n Experimental study.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Venous blood was collected from 8 healthy horses and 8 horses with pituitary pars intermedia dysfunction (\n PPID<\/jats:styled-content>\n ) with a range of\n ACTH<\/jats:styled-content>\n concentrations. Baseline\n ACTH<\/jats:styled-content>\n concentrations were established immediately using a chemiluminescent assay. Plasma samples were then: 1) centrifuged immediately, 2) centrifuged immediately with the addition of maleimide, or 3) allowed to separate by gravity followed by the addition of maleimide, before all samples were stored at 22\u00b0C and analysed at 4, 8, 24 and 48\u2009h post collection. A linear mixed effects model and Bland\u2010Altman analyses were performed. Significance was set at P < 0.05.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n No significant effect of plasma treatment (P = 0.1) on change in\n ACTH<\/jats:styled-content>\n concentration was identified. However, significant effects of horse health status (P<0.001) and time (P<0.001) on change in\n ACTH<\/jats:styled-content>\n concentration were identified. No significant interactions were found. Significant decreases in\n ACTH<\/jats:styled-content>\n concentration occurred in horses with\n PPID<\/jats:styled-content>\n between 4 and 8\u2009h after blood collection. In non\u2010\n PPID<\/jats:styled-content>\n horses, the decrease in\n ACTH<\/jats:styled-content>\n concentration over time was not significant. Agreement with baseline values decreased over time and was greater for non\u2010\n PPID<\/jats:styled-content>\n horses than for\n PPID<\/jats:styled-content>\n horses.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Clinically useful results are still obtained if\n ACTH<\/jats:styled-content>\n concentration is measured up to 48\u2009h after sample collection. Allowing samples to separate by gravity rather than centrifugation did not have a significant effect on\n ACTH<\/jats:styled-content>\n concentration, and the addition of maleimide was of no benefit.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/evj.12319","type":"journal-article","created":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T20:42:24Z","timestamp":1404247344000},"page":"587-591","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The effects of sample handling and\n \n N<\/scp>\n <\/i>\n \u2010phenylmaleimide on concentration of adrenocorticotrophic hormone in equine plasma"],"prefix":"10.1111","volume":"47","author":[{"given":"D. I.","family":"Rendle","sequence":"first","affiliation":[{"name":"School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Litchfield","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Gough","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Cowling","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. J.","family":"Hughes","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2012.00578.x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2010.12.007"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.45-1-26"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516402776250333"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.1996.tb02016.x"},{"key":"e_1_2_11_7_1","first-page":"63","article-title":"Studies on the stability of human adrenocorticotrophin in blood and plasma","volume":"71","author":"Hogan P.","year":"1976","journal-title":"J. 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