{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T09:38:37Z","timestamp":1785577117099,"version":"3.56.0"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,8,21]],"date-time":"2015-08-21T00:00:00Z","timestamp":1440115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2016,7]]},"abstract":"Summary<\/jats:title>\n \n Reasons for performing study<\/jats:title>\n \n The role of equid \u03b3\u2010herpesviruses on ocular surface diseases has been disputed, because the diagnosis is usually based on clinical symptoms and detection of viral\n DNA<\/jats:styled-content>\n from samples obtained from live animals.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n To describe the clinical course, results of polymerase chain reaction (PCR) analysis,\n in\u00a0situ<\/jats:italic>\n hybridisation, cell culture and pathohistological findings of select cases in a presumed outbreak of herpesvirus infection in a group of 15 Icelandic horses.\n <\/jats:p>\n <\/jats:sec>\n \n Study design<\/jats:title>\n Case series.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Pooled ocular and nasal swabs and peripheral blood mononuclear cells of horses diagnosed clinically with herpesvirus\u2010associated keratoconjunctivitis were analysed for presence of equine herpesviruses (EHV)\u20102 and\n EHV<\/jats:styled-content>\n \u20105 nucleic acid using real\u2010time\n PCR<\/jats:styled-content>\n . Necropsy specimens from one horse, subjected to euthanasia due to deterioration of clinical symptoms were examined histopathologically, and analysed for presence of\n EHV<\/jats:styled-content>\n \u20102 and\n EHV<\/jats:styled-content>\n \u20105 nucleic acid using real\u2010time\n PCR<\/jats:styled-content>\n .\n In situ<\/jats:italic>\n hybridisation and cell culture of select samples were performed.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n All horses with symptoms of severe keratoconjunctivitis were positive for presence of either\n EHV<\/jats:styled-content>\n \u20102 and\/or\n EHV<\/jats:styled-content>\n \u20105 nucleic acid using real\u2010time\n PCR<\/jats:styled-content>\n . Assessment of necropsy specimens of the most severely affected case, revealed presence of\n EHV<\/jats:styled-content>\n \u20102 and\/or\n EHV<\/jats:styled-content>\n \u20105 nucleic acid in several ocular and extraocular anatomical locations. The remaining horses responded favourably to symptomatic treatment.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n This case series illustrates a severe outbreak of keratoconjunctivitis in a group of Icelandic horses, with suspected \u03b3\u2010herpesvirus involvement. For the first time equid \u03b3\u2010herpesviruses were detected in intraocular anatomical locations.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/evj.12465","type":"journal-article","created":{"date-parts":[[2015,6,2]],"date-time":"2015-06-02T01:55:24Z","timestamp":1433210124000},"page":"427-429","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Keratoconjunctivitis in a group of Icelandic horses with suspected \u03b3\u2010herpesvirus involvement"],"prefix":"10.1111","volume":"48","author":[{"given":"J. O.","family":"Rushton","sequence":"first","affiliation":[{"name":"Department of Companion Animals and Horses University of Veterinary Medicine Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Kolodziejek","sequence":"additional","affiliation":[{"name":"Institute of Virology University of Veterinary Medicine Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Nell","sequence":"additional","affiliation":[{"name":"Department of Companion Animals and Horses University of Veterinary Medicine Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Weissenb\u00f6ck","sequence":"additional","affiliation":[{"name":"Institute of Pathology and Forensic Veterinary Medicine University of Veterinary Medicine Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Nowotny","sequence":"additional","affiliation":[{"name":"Institute of Virology University of Veterinary Medicine Vienna Austria"},{"name":"Department of Microbiology and Immunology College of Medicine and Health Sciences Sultan Qaboos University Muscat Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"329","DOI":"10.2460\/javma.1994.205.02.329","article-title":"Isolation of equine herpesvirus type 2 (equine gammaherpesvirus 2) from foals with keratoconjunctivitis","volume":"205","author":"Collinson P.N.","year":"1994","journal-title":"J. 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