{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T15:48:41Z","timestamp":1762098521575,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"S48","license":[{"start":{"date-parts":[[2015,9,6]],"date-time":"2015-09-06T00:00:00Z","timestamp":1441497600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2015,9]]},"abstract":"\n Reasons for performing study<\/jats:title>\n \n Hypoglycin A (\n HG<\/jats:styled-content>\n ) appears to cause atypical myopathy (\n AM<\/jats:styled-content>\n ), but to our knowledge, detection of\n HG<\/jats:styled-content>\n in affected and unaffected horses and concurrently in plants that they were exposed to has not previously been reported.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n To investigate\n HG<\/jats:styled-content>\n in samples from horses exposed to\n \n A<\/jats:styled-content>\n cer pseudoplatanus\n <\/jats:italic>\n (European sycamore maple) and in such plant material, at the time of clinical cases of\n AM<\/jats:styled-content>\n in the herd.\n <\/jats:p>\n <\/jats:sec>\n \n Study design<\/jats:title>\n Cross\u2010sectional study.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Blood was collected from 2 horses with\n AM<\/jats:styled-content>\n and 22 clinically healthy co\u2010grazing horses in 2 Swedish farms within one week of onset of signs (\n M<\/jats:styled-content>\n ay 2014) and one month later, after horses were moved to other pastures. Ten healthy control horses from unaffected farms were sampled once. Samaras, seedlings, flowers and leaves from\n \n A<\/jats:styled-content>\n cer pseudoplatanus\n <\/jats:italic>\n and from\n \n A<\/jats:styled-content>\n cer platanoides L\n <\/jats:italic>\n (\n N<\/jats:styled-content>\n orway maple) were collected from affected pastures. Hypoglycin A was analysed using chemical derivatisation with dansyl chloride (\n DNS<\/jats:styled-content>\n ) and ultra high performance liquid chromatography\u2013tandem mass spectrometry. Hypoglycin A was detected as derivatised compound\n HG\u2010DNS<\/jats:styled-content>\n [M+\n H<\/jats:styled-content>\n ]+ with selected reaction monitoring.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Hypoglycin A was detected in the horses affected with\n AM<\/jats:styled-content>\n , and also in 20 out of 22 co\u2010grazing horses. One month later, a surviving case horse and 9\/20 co\u2010grazing horses were still positive for\n HG<\/jats:styled-content>\n . Controls from other farms were negative for\n HG<\/jats:styled-content>\n . Hypoglycin A was detected in plant material from\n \n A<\/jats:styled-content>\n cer pseudoplatanus\n <\/jats:italic>\n , but not from\n \n A<\/jats:styled-content>\n cer platanoides L\n <\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Horses grazing in pastures with\n HG<\/jats:styled-content>\n \u2010containing\n \n A<\/jats:styled-content>\n cer pseudoplatanus\n <\/jats:italic>\n were positive for\n HG<\/jats:styled-content>\n in blood, and some showed severe signs of myopathy.\n <\/jats:p>\n \n Ethical animal research:<\/jats:bold>\n \u2002Ethical consent for blood sampling was granted (C113\/11) and horse owners gave their informed consent to inclusion of horses in the study.\n Source of funding:<\/jats:bold>\n \u2002National Veterinary Institute,\n S<\/jats:styled-content>\n weden.\n Competing interests:<\/jats:bold>\n \u2002None declared.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/evj.12486_49","type":"journal-article","created":{"date-parts":[[2015,9,7]],"date-time":"2015-09-07T00:26:36Z","timestamp":1441585596000},"page":"22-22","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Detection of the Toxin Hypoglycin A in Pastured Horses and in the European Sycamore Maple Tree (\n \n A<\/scp>\n cer Pseudoplatanus\n <\/i>\n ) During Two Outbreaks of Atypical Myopathy in\n S<\/scp>\n weden"],"prefix":"10.1111","volume":"47","author":[{"given":"G.","family":"Gr\u00f6ndahl","sequence":"first","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Berglund","sequence":"additional","affiliation":[{"name":"Distriktsveterin\u00e4rerna Flyinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Skidell","sequence":"additional","affiliation":[{"name":"Evidensia Specialist Horse Hospital Helsingborg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Bondesson","sequence":"additional","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Salomonsson","sequence":"additional","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,6]]},"container-title":["Equine Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fevj.12486_49","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/evj.12486_49","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T08:40:46Z","timestamp":1762072846000},"score":1,"resource":{"primary":{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/10.1111\/evj.12486_49"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":0,"journal-issue":{"issue":"S48","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1111\/evj.12486_49"],"URL":"https:\/\/doi.org\/10.1111\/evj.12486_49","archive":["Portico"],"relation":{},"ISSN":["0425-1644","2042-3306"],"issn-type":[{"type":"print","value":"0425-1644"},{"type":"electronic","value":"2042-3306"}],"subject":[],"published":{"date-parts":[[2015,9]]},"assertion":[{"value":"2015-09-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}