{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T08:43:54Z","timestamp":1776847434056,"version":"3.51.2"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,1,16]],"date-time":"2016-01-16T00:00:00Z","timestamp":1452902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001655","name":"Grayson-Jockey Club Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001655","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010291","name":"United States Equestrian Federation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010291","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["2\u2010P30\u2010DK047757\u201016"],"award-info":[{"award-number":["2\u2010P30\u2010DK047757\u201016"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Reasons for performing study<\/jats:title>\n                    <jats:p>Our long\u2010term aim is to develop a gene therapy approach for the prevention of laminitis in the contralateral foot of horses with major musculoskeletal injuries and non\u2010weightbearing lameness.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The goal of this study was to develop a practical method to efficiently deliver therapeutic proteins deep within the equine foot.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>\n                      Randomised\n                      <jats:italic>in vivo<\/jats:italic>\n                      experiment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used recombinant adeno\u2010associated viral vectors (rAAVs) to deliver marker genes using regional limb perfusion through the palmar digital artery of the horse.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Vector serotypes rAAV2\/1, 2\/8 and 2\/9 all successfully transduced equine foot tissues and displayed similar levels and patterns of transduction. The regional distribution of transduction within the foot decreased with decreasing vector dose. The highest transduction values were seen in the sole and coronary regions and the lowest transduction values were detected in the dorsal hoof\u2010wall region. The use of a surfactant\u2010enriched vector diluent increased regional distribution of the vector and improved the transduction in the hoof\u2010wall region. The hoof\u2010wall region of the foot, which exhibited the lowest levels of transduction using saline as the vector diluent, displayed a dramatic increase in transduction when surfactant was included in the vector diluent (9\u2010 to 81\u2010fold increase). In transduced tissues, no significant difference was observed between promoters (chicken \u03b2\u2010actin vs. cytomegalovirus) for gene expression. All horses tested for vector\u2010neutralising antibodies were positive for serotype\u2010specific neutralising antibodies to rAAV2\/5.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The current experiments demonstrate that transgenes can be successfully delivered to the equine distal extremity using rAAV vectors and that serotypes 2\/8, 2\/9 and 2\/1 can successfully transduce tissues of the equine foot. When the vector was diluted with surfactant\u2010containing saline, the level of transduction increased dramatically. The increased level of transduction due to the addition of surfactant also improved the distribution pattern of transduction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.12547","type":"journal-article","created":{"date-parts":[[2015,12,11]],"date-time":"2015-12-11T03:15:17Z","timestamp":1449803717000},"page":"79-86","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Delivery and evaluation of recombinant adeno\u2010associated viral vectors in the equine distal extremity for the treatment of laminitis"],"prefix":"10.1111","volume":"49","author":[{"given":"J. B.","family":"Mason","sequence":"first","affiliation":[{"name":"Department of Clinical Studies New Bolton Center School of Veterinary Medicine University of Pennsylvania Kennett Square USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. L.","family":"Gurda","sequence":"additional","affiliation":[{"name":"Gene Therapy Program Department of Pathology and Laboratory Medicine University of Pennsylvania Philadelphia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Van Wettere","sequence":"additional","affiliation":[{"name":"Utah Veterinary Diagnostic Laboratory School of Veterinary Medicine Utah State University Logan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. B.","family":"Engiles","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies New Bolton Center School of Veterinary Medicine University of Pennsylvania Kennett Square USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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