{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:00:19Z","timestamp":1771632019855,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,2,29]],"date-time":"2016-02-29T00:00:00Z","timestamp":1456704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Technology Innovation Agency, Pretoria, South Africa"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Reasons for performing study<\/jats:title>\n                    <jats:p>\n                      Few studies have investigated ovarian function in the mare undergoing porcine zona pellucida (\n                      <jats:styled-content style=\"fixed-case\">pZP<\/jats:styled-content>\n                      ) immunocontraception despite reported ovarian dysfunction in other species.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      This study aimed to describe ovarian function and oestrous cyclicity in pony mares following treatment with either the conventional\n                      <jats:styled-content style=\"fixed-case\">pZP<\/jats:styled-content>\n                      vaccine or a novel recombinant form of the vaccine derived from porcine\n                      <jats:styled-content style=\"fixed-case\">ZP<\/jats:styled-content>\n                      3 and\n                      <jats:styled-content style=\"fixed-case\">ZP<\/jats:styled-content>\n                      4 (re\n                      <jats:styled-content style=\"fixed-case\">ZP<\/jats:styled-content>\n                      ). In addition, the contraceptive efficacy of\n                      <jats:styled-content style=\"fixed-case\">pZP<\/jats:styled-content>\n                      vs. re\n                      <jats:styled-content style=\"fixed-case\">ZP<\/jats:styled-content>\n                      was assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Blinded, randomised, prospective clinical trial.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Mares (n = 21) were randomised into 3 groups of 7:\n                      <jats:italic>Group I<\/jats:italic>\n                      received the\n                      <jats:styled-content style=\"fixed-case\">pZP<\/jats:styled-content>\n                      vaccine, with a booster 5 weeks later;\n                      <jats:italic>\n                        Group\n                        <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\n                      <\/jats:italic>\n                      received the re\n                      <jats:styled-content style=\"fixed-case\">ZP<\/jats:styled-content>\n                      vaccine, with a booster 5 weeks later; and\n                      <jats:italic>\n                        Group\n                        <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>\n                      <\/jats:italic>\n                      (controls) received 2 treatments, 5 weeks apart, of saline and adjuvant alone. Mares underwent weekly monitoring via transrectal palpation and ultrasound examination of the reproductive tract, with daily monitoring during oestrus. Data were collected over a 24 week period coinciding with the physiological breeding season; treatments commenced in Week 4. Serum samples were obtained for antibody titres and ovarian steroid level analyses at 7 day intervals. Cycling mares were bred via fresh semen artificial inseminations over a maximum of 2 consecutive oestrous cycles, commencing 5 weeks post booster vaccination.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Control mares cycled throughout the trial. After treatment, 6 of 7\n                      <jats:styled-content style=\"fixed-case\">pZP<\/jats:styled-content>\n                      mares (86%) and one re\n                      <jats:styled-content style=\"fixed-case\">ZP<\/jats:styled-content>\n                      mare (14%) had an extended anoestrus that correlated with basal serum oestradiol and progesterone levels. All mares resumed cyclicity by 10 months post treatment. Pregnancies were diagnosed in all controls, 4 re\n                      <jats:styled-content style=\"fixed-case\">ZP<\/jats:styled-content>\n                      \u2010 (57%) and none of the\n                      <jats:styled-content style=\"fixed-case\">pZP<\/jats:styled-content>\n                      \u2010immunised mares.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The current study demonstrates the reversible suppression of ovarian function in pony mares following treatment with\n                      <jats:styled-content style=\"fixed-case\">pZP<\/jats:styled-content>\n                      . The effect of the re\n                      <jats:styled-content style=\"fixed-case\">ZP<\/jats:styled-content>\n                      vaccine on pregnancy outcome requires further investigation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.12557","type":"journal-article","created":{"date-parts":[[2015,12,26]],"date-time":"2015-12-26T10:01:13Z","timestamp":1451124073000},"page":"189-195","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Ovarian function and pregnancy outcome in pony mares following immunocontraception with native and recombinant porcine zona pellucida vaccines"],"prefix":"10.1111","volume":"49","author":[{"given":"C. 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