{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T16:04:08Z","timestamp":1785600248381,"version":"3.56.0"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,5,22]],"date-time":"2016-05-22T00:00:00Z","timestamp":1463875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Sexing Technologies, USA and EquiBreed NZ Ltd"},{"name":"Postgraduate Research Scholarship from the Australian Rural Industries Research and Development\nCorporation"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Reasons for performing study<\/jats:title>\n                    <jats:p>The fertility of sex\u2010sorted, cryopreserved stallion sperm must be improved for the sex\u2010sorting technology to be applied commercially.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To optimise the conditions used to liquid store stallion sperm prior to sex\u2010sorting and assess the fertility of sperm following sex\u2010sorting and cryopreservation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>\n                      Both\n                      <jats:italic>in vitro<\/jats:italic>\n                      experiment and randomised controlled trial in healthy, client\u2010owned mares.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Stallion ejaculates (n = 9) were diluted in either a skimmed milk (\n                      <jats:styled-content style=\"fixed-case\">KMT<\/jats:styled-content>\n                      ) or\n                      <jats:styled-content style=\"fixed-case\">BSA<\/jats:styled-content>\n                      (I\u2010\n                      <jats:styled-content style=\"fixed-case\">BSA<\/jats:styled-content>\n                      ) based media to 25 \u00d7 10\n                      <jats:sup>6<\/jats:sup>\n                      sperm\/ml directly (+\n                      <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>\n                      25) or washed to remove seminal plasma and diluted to 25 or 111 \u00d7 10\n                      <jats:sup>6<\/jats:sup>\n                      sperm\/ml (\u2010\n                      <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>\n                      25 and \u2010\n                      <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>\n                      111). Sperm were stored for 18 h at 10 to 15\u00b0C and \u2010\n                      <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>\n                      25 and +\n                      <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>\n                      25 treatments were centrifuged and resuspended to 111 \u00d7 10\n                      <jats:sup>6<\/jats:sup>\n                      sperm\/ml. Sperm were incubated under H33342 staining conditions and motility, viability and acrosome integrity assessed. Semen was collected from stallions (n = 4), liquid stored at 10\u201315\u00b0C for up to 5 h and sperm either cryopreserved directly, sex\u2010sorted and cryopreserved, or sex\u2010sorted and returned to liquid storage until insemination. Low\u2010dose hysteroscopic insemination was performed in 23 mares randomly allocated to the semen preparation group and pregnancy determined following embryo flushing on Day 9 after ovulation, or via transrectal ultrasonography on Day 14 after ovulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Skimmed milk was superior to I\u2010\n                      <jats:styled-content style=\"fixed-case\">BSA<\/jats:styled-content>\n                      in maintaining motility, viability and acrosome integrity. Seminal plasma removal did not affect the parameters measured at the concentrations examined. Conception rates did not differ significantly between the groups, although a high incidence of pregnancy loss was observed in both the cryopreserved groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>While the conception rates achieved are among the highest yet reported for sex\u2010sorted, cryopreserved stallion sperm, the high incidence of pregnancy loss suggests that the development of the resulting embryos was significantly impaired by the sperm processing treatments.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.12583","type":"journal-article","created":{"date-parts":[[2016,4,10]],"date-time":"2016-04-10T14:09:53Z","timestamp":1460297393000},"page":"160-166","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Field fertility of liquid stored and cryopreserved flow cytometrically sex\u2010sorted stallion sperm"],"prefix":"10.1111","volume":"49","author":[{"given":"Z.","family":"Gibb","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Science The University of Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. G.","family":"Grupen","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science The University of Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. M. C.","family":"Maxwell","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science The University of Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. H. A.","family":"Morris","sequence":"additional","affiliation":[{"name":"EquiBreed NZ Limited Te Awamutu New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.08.015"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516402776767321"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516402776767178"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2003.11.008"},{"key":"e_1_2_11_6_1","first-page":"510","article-title":"Effects of caffeine stimulation on stallion sperm motion characteristics following 18\u2010H storage, flow\u2010sorting, and cryopreservation","volume":"59","author":"Lindsey A.C.","year":"2003","journal-title":"Theriogenol."},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0737-0806(00)80135-5"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.2006.00882.x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.05.030"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(01)00154-3"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD03060"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.10.001"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.06.032"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.01.013"},{"key":"e_1_2_11_15_1","first-page":"97","volume-title":"Salamon's Artificial Insemination of Sheep and Goats","author":"Evans G.","year":"1987"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.024"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.2527\/1991.6983308x"},{"key":"e_1_2_11_18_1","first-page":"83","article-title":"Improved flow sorting resolution of X and Y chromosomes bearing viable sperm separation using dual staining and dead cell gating","volume":"17","author":"Johnson L.A.","year":"1994","journal-title":"Cytometry"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.1991.tb01546.x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(97)00035-6"},{"key":"e_1_2_11_21_1","first-page":"47","article-title":"Centrifugation of stallion semen and its storage in large volume straws","volume":"27","author":"Martin J.C.","year":"1979","journal-title":"J. Reprod. Fertil."},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1180095"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1230445"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01314.x"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2007.02.008"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00444-7"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.05.087"},{"key":"e_1_2_11_28_1","first-page":"16","volume-title":"Havemeyer Foundation Workshop: From Epididymis to Embryo","author":"Squires E.L.","year":"2001"},{"key":"e_1_2_11_29_1","first-page":"512","article-title":"The longevity and acrosome status of stallion flow sorted spermatozoa","volume":"59","author":"Morris L.H.A.","year":"2003","journal-title":"Theriogenol"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516402776181222"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0264.2007.00795.x"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00108-9"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1134"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00225-3"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1530\/ror.0.0040031"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2000-12552"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2010.00565.x"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.10.021"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD98102"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.07.008"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.01.063"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-08-0484"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(03)00113-4"}],"container-title":["Equine Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fevj.12583","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fevj.12583","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/evj.12583","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/evj.12583","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/evj.12583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:22:21Z","timestamp":1762078941000},"score":1,"resource":{"primary":{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/10.1111\/evj.12583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,22]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1111\/evj.12583"],"URL":"https:\/\/doi.org\/10.1111\/evj.12583","archive":["Portico"],"relation":{},"ISSN":["0425-1644","2042-3306"],"issn-type":[{"value":"0425-1644","type":"print"},{"value":"2042-3306","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,22]]},"assertion":[{"value":"2015-08-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-03-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-05-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}