{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T22:37:09Z","timestamp":1776465429447,"version":"3.51.2"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T00:00:00Z","timestamp":1476316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nutriquine NV"},{"name":"Sonac BV"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2017,7]]},"abstract":"Summary<\/jats:title>\n \n Background<\/jats:title>\n Nutraceuticals are often used in the management of equine osteoarthritis, but scientific evidence of their efficacy is lacking.<\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n To study the preventive effects of two new nutraceuticals after the experimental induction of synovitis in comparison with positive and negative control treatments.<\/jats:p>\n <\/jats:sec>\n \n Study design<\/jats:title>\n Blinded, controlled, randomised experiment.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Twenty\u2010four healthy Standardbred horses were randomly allocated to supplement\n AT<\/jats:styled-content>\n (multi\u2010ingredient, 28 days), supplement\n HP<\/jats:styled-content>\n (collagen hydrolysate, 60 days), meloxicam (4 days) or placebo (60 days). Synovitis was induced in the right intercarpal joint by intra\u2010articular injection of 0.5 ng lipopolysaccharide (\n LPS<\/jats:styled-content>\n ) of\n Escherichia coli<\/jats:italic>\n while treatments were continued. Blood and synovial fluid were sampled before treatment, immediately prior to\n LPS<\/jats:styled-content>\n injection, and at 8, 24 and 48 h post\u2010injection. Synovial fluid samples were analysed for total nucleated cell count (\n TNCC<\/jats:styled-content>\n ), total protein (\n TP<\/jats:styled-content>\n ) and selected biomarkers (prostaglandin E\n 2<\/jats:sub>\n [\n PGE<\/jats:styled-content>\n 2<\/jats:sub>\n ], interleukin\u20106 [\n IL<\/jats:styled-content>\n \u20106], glycosaminoglycans [\n GAG<\/jats:styled-content>\n s], type II collagen synthesis [\n CPII<\/jats:styled-content>\n ], matrix metalloproteinase [\n MMP<\/jats:styled-content>\n ]). Lameness was scored by visual examination and pressure plate analysis immediately prior to\n LPS<\/jats:styled-content>\n injection, and at 8, 24 and 48 h post\u2010injection. Clinical examinations were performed before treatment, immediately prior to\n LPS<\/jats:styled-content>\n injection, at 2, 4 and 6 h post\u2010injection, and then twice per day during the test period.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Before treatment and intra\u2010articular challenge, there were no statistically significant differences among the treatment groups for any of the parameters. After intra\u2010articular challenge, the placebo group showed significantly higher synovial fluid\n TP<\/jats:styled-content>\n ,\n TNCC<\/jats:styled-content>\n and\n PGE<\/jats:styled-content>\n 2<\/jats:sub>\n compared with the meloxicam group, although the model did not induce a relevant amount of lameness. Both nutraceuticals resulted in significantly lower synovial fluid\n TP<\/jats:styled-content>\n ,\n TNCC<\/jats:styled-content>\n and\n PGE<\/jats:styled-content>\n 2<\/jats:sub>\n compared with placebo. No statistical differences in\n IL<\/jats:styled-content>\n \u20106,\n GAG<\/jats:styled-content>\n s,\n CPII<\/jats:styled-content>\n or\n MMP<\/jats:styled-content>\n s were observed among treatment groups. No adverse effects were observed.\n <\/jats:p>\n <\/jats:sec>\n \n Main limitations<\/jats:title>\n Despite evidence of synovitis, lameness was too mild to detect.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n The preventive administration of these nutraceuticals showed anti\u2010inflammatory effects in this validated synovitis model. Therefore, further studies of their clinical applicability are warranted.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/evj.12629","type":"journal-article","created":{"date-parts":[[2016,8,24]],"date-time":"2016-08-24T05:33:20Z","timestamp":1472016800000},"page":"532-538","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["The preventive effects of two nutraceuticals on experimentally induced acute synovitis"],"prefix":"10.1111","volume":"49","author":[{"given":"E.","family":"van de Water","sequence":"first","affiliation":[{"name":"Department of Surgery and Anaesthesiology Faculty of Veterinary Medicine Ghent University Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Oosterlinck","sequence":"additional","affiliation":[{"name":"Department of Surgery and Anaesthesiology Faculty of Veterinary Medicine Ghent University Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Dumoulin","sequence":"additional","affiliation":[{"name":"Department of Surgery and Anaesthesiology Faculty of Veterinary Medicine Ghent University Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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R.","family":"van Weeren","sequence":"additional","affiliation":[{"name":"Department of Equine Sciences Faculty of Veterinary Medicine Utrecht University The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"van den Broek","sequence":"additional","affiliation":[{"name":"Department of Farm Animal Health Faculty of Veterinary Medicine Utrecht University The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Everts","sequence":"additional","affiliation":[{"name":"Department of Farm Animal Health Faculty of Veterinary Medicine Utrecht University The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Pille","sequence":"additional","affiliation":[{"name":"Department of Surgery and Anaesthesiology Faculty of Veterinary Medicine Ghent University Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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