{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T02:40:57Z","timestamp":1785465657690,"version":"3.56.0"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,1,12]],"date-time":"2017-01-12T00:00:00Z","timestamp":1484179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Unlike in Warmblood horses, aortic rupture is quite common in Friesian horses, in which a hereditary trait is suspected. The aortic connective tissue in affected Friesians shows histological changes such as medial necrosis, elastic fibre fragmentation, mucoid material accumulation and fibrosis with aberrant collagen morphology. However, ultrastructural examination of the collagen fibres of the mid\u2010thoracic aorta has been inconclusive in further elucidating the pathogenesis of the disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To assess several extracellular matrix (\n                      <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>\n                      ) components biochemically in order to explore a possible underlying breed\u2010related systemic\n                      <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>\n                      defect in Friesians with aortic rupture.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Cadaver study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Tissues from affected Friesians (n = 18), unaffected Friesians (n = 10) and Warmblood horses (n = 30) were compared. Samples were taken from the thoracic aorta at the level of the rupture site, from two locations caudal to the rupture and from the deep digital flexor tendon. Total collagen content, post\u2010translational modifications of collagen formation including lysine hydroxylation, and hydroxylysylpyridinoline (\n                      <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>\n                      ), lysylpyridinoline (\n                      <jats:styled-content style=\"fixed-case\">LP<\/jats:styled-content>\n                      ) and pyrrole cross\u2010links were analysed. Additionally, elastin cross\u2010links, glycosaminoglycan content and matrix metalloproteinase (\n                      <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\n                      ) activity were assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Significantly increased\n                      <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\n                      activity and increased\n                      <jats:styled-content style=\"fixed-case\">LP<\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>\n                      cross\u2010linking, lysine hydroxylation and elastin cross\u2010linking were found at the site of rupture in affected Friesians. These changes may reflect processes involved in healing and aneurysm formation. Unaffected Friesians had less lysine hydroxylation and pyrrole cross\u2010linking within the tendons compared with Warmblood horses. No differences in the matrix of the aorta were found between normal Warmbloods and Friesian horses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitations<\/jats:title>\n                    <jats:p>Small sample size.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The differences in collagen parameters in tendon tissue may reflect differences in connective tissue metabolism between Friesians and Warmblood horses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.12654","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T14:39:11Z","timestamp":1479307151000},"page":"609-613","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Differences in extracellular matrix proteins between Friesian horses with aortic rupture, unaffected Friesians and Warmblood horses"],"prefix":"10.1111","volume":"49","author":[{"given":"M.","family":"Ploeg","sequence":"first","affiliation":[{"name":"Department of Pathobiology Faculty of Veterinary Medicine Utrecht University Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Gr\u00f6ne","sequence":"additional","affiliation":[{"name":"Department of Pathobiology Faculty of Veterinary Medicine Utrecht University Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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A.","family":"van de Lest","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Biology Faculty of Veterinary Medicine Utrecht University Utrecht the Netherlands"},{"name":"Department of Equine Sciences Faculty of Veterinary Medicine Utrecht University Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Saey","sequence":"additional","affiliation":[{"name":"Department of Pathology, Bacteriology and Poultry Diseases Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Duchateau","sequence":"additional","affiliation":[{"name":"Department of Comparative Physiology and Biometrics Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Wolsein","sequence":"additional","affiliation":[{"name":"Institute for Pathology University of Veterinary Medicine Foundation Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Chiers","sequence":"additional","affiliation":[{"name":"Department of Pathology, Bacteriology and Poultry Diseases Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Ducatelle","sequence":"additional","affiliation":[{"name":"Department of Pathology, Bacteriology and Poultry Diseases Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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