{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:46:00Z","timestamp":1789479960507,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,2,28]],"date-time":"2017-02-28T00:00:00Z","timestamp":1488240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001862","name":"Svenska Forskningsr\u00e5det Formas","doi-asserted-by":"publisher","award":["FORMAS 221\u20102013\u2010317"],"award-info":[{"award-number":["FORMAS 221\u20102013\u2010317"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Clinical tools to diagnose the early changes of osteoarthritis (\n                      <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>\n                      ) that occur in the articular cartilage are lacking.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      We sought to identify and quantify a novel cartilage oligomeric matrix protein (\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      ) neoepitope in the synovial fluid from the joints of healthy horses and those with different stages of\n                      <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>\n                      <jats:italic>In vitro<\/jats:italic>\n                      quantitative proteomics and assay development with application in synovial fluids samples obtained from biobanks of well\u2010characterised horses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Articular cartilage explants were incubated with or without interleukin\u20101\u03b2 for 25 days. Media were analysed via quantitative proteomics. Synovial fluid was obtained from either normal joints (n = 15) or joints causing lameness (n = 17) or with structural\n                      <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>\n                      lesions (n = 7) and analysed for concentrations of the\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neoepitope using a custom\u2010developed inhibition enzyme\u2010linked immunosorbent assay (\n                      <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\n                      ). Explants were immunostained with polyclonal antibodies against\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      and the\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neoepitopes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Semitryptic\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      peptides were identified and quantified in cell culture media from cartilage explants. A rabbit polyclonal antibody was raised against the neoepitope of the N\u2010terminal portion of one\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      fragment (sequence\n                      <jats:styled-content style=\"fixed-case\">SGPTHEGVC<\/jats:styled-content>\n                      ). An inhibition\n                      <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\n                      was developed to quantify the\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neoepitope in synovial fluid. The mean concentration of the\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neoepitope significantly increased in the synovial fluid from the joints responsible for acute lameness compared with normal joints and the joints of chronically lame horses and in joints with chronic structural\n                      <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>\n                      . Immunolabelling for the\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neoepitope revealed a pericellular staining in the interleukin\u20101\u03b2\u2010stimulated explants.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitations<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\n                      is based on polyclonal antisera rather than a monoclonal antibody.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The increase in the\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neoepitope in the synovial fluid from horses with acute lameness suggests that this neoepitope has the potential to be a unique candidate biomarker for the early molecular changes in articular cartilage associated with\n                      <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.12666","type":"journal-article","created":{"date-parts":[[2017,1,17]],"date-time":"2017-01-17T21:14:01Z","timestamp":1484687641000},"page":"662-667","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Cartilage oligomeric matrix protein neoepitope in the synovial fluid of horses with acute lameness: A new biomarker for the early stages of osteoarthritis"],"prefix":"10.1111","volume":"49","author":[{"given":"E.","family":"Ski\u00f6ldebrand","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry and Transfusion Medicine Institute of Biomedicine Sahlgrenska University Hospital Gothenburg University Gothenburg Sweden"},{"name":"Division of Pathology, Pharmacology and Toxicology Department of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ekman","sequence":"additional","affiliation":[{"name":"Division of Pathology, Pharmacology and Toxicology Department of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Mattsson Hult\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry Sahlgrenska University Hospital Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Svala","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Transfusion Medicine Institute of Biomedicine Sahlgrenska University Hospital Gothenburg University Gothenburg Sweden"},{"name":"Division of Pathology, Pharmacology and Toxicology Department of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Bj\u00f6rkman","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry Sahlgrenska University Hospital Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lindahl","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Transfusion Medicine Institute of Biomedicine Sahlgrenska University Hospital Gothenburg University Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lundqvist","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry Sahlgrenska University Hospital Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"\u00d6nnerfjord","sequence":"additional","affiliation":[{"name":"Section for Rheumatology and Molecular Skeletal Biology Department of Clinical Sciences Lund Lund University Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Sihlbom","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility Sahlgrenska Academy Gothenburg University Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"R\u00fcetschi","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Transfusion Medicine Institute of Biomedicine Sahlgrenska University Hospital Gothenburg University Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2005.36471"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2002.36241"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2010.07.007"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.198"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1693"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-014-0429-9"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/2229-3485.148792"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0b013e328349c2b1"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.554683"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22963"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.511972"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008207.2015.1027340"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516401776249480"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.2006.00877.x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-8261.2003.tb00498.x"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.72.9.1156"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780350404"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(03)00034-9"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(02)00015-X"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.32.20397"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009512200"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504778200"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.234716"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2013.03.007"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2014.06.001"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2014.04.030"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2006.04.001"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2015.03.002"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2013.08.012"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2015.04.004"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2010.04.012"}],"container-title":["Equine Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fevj.12666","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/evj.12666","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/evj.12666","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/evj.12666","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:00:58Z","timestamp":1762077658000},"score":1,"resource":{"primary":{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/10.1111\/evj.12666"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,28]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/evj.12666"],"URL":"https:\/\/doi.org\/10.1111\/evj.12666","archive":["Portico"],"relation":{},"ISSN":["0425-1644","2042-3306"],"issn-type":[{"value":"0425-1644","type":"print"},{"value":"2042-3306","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,28]]},"assertion":[{"value":"2016-06-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}