{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T14:00:21Z","timestamp":1762956021056,"version":"build-2065373602"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T00:00:00Z","timestamp":1551830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["VR 2015\u201002959"],"award-info":[{"award-number":["VR 2015\u201002959"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001862","name":"Svenska Forskningsr\u00e5det Formas","doi-asserted-by":"publisher","award":["FORMAS 221\u20102013\u2010317"],"award-info":[{"award-number":["FORMAS 221\u20102013\u2010317"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Molecular serum markers that can identify early reversible osteoarthritis (\n                      <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>\n                      ) in horses are lacking.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      We studied serum concentrations of a novel cartilage oligomeric matrix protein (\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      ) neo\u2010epitope in horses subjected to short\u2010term exercise and with acute lameness. The effects of circadian rhythm and age were also evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Longitudinal studies in healthy horses and cross\u2010sectional comparison of lame and non\u2010lame horses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Sera were collected from five horses before and after short\u2010term interval exercise and during full\u2010day box rest. Sera from 32 acutely lame horses were used to evaluate age\u2010related effects. Independent samples from control horses (n = 41) and horses with acute lameness (n = 71) were included.\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neo\u2010epitope concentrations were analysed using custom\u2010developed inhibition\n                      <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\n                      s validated for equine serum. The presence of\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neo\u2010epitope was delineated in healthy and osteoarthritic articular cartilage with immunohistochemistry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neo\u2010epitope concentrations decreased after speed training but returned to baseline levels post\u2010exercise. No correlations between age and serum\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neo\u2010epitope concentrations were found (r = 0.0013). The mean (\u00b1s.d.) serum concentration of\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neo\u2010epitope in independent samples from non\u2010lame horses was 0.84 \u00b1 0.38 \u03bcg\/mL, and for lame horses was 5.24 \u00b1 1.83 \u03bcg\/mL (P&lt;0.001). Antibodies against\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neo\u2010epitope did not stain normal articular cartilage, but intracytoplasmic staining was found in superficial chondrocytes of mild\n                      <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>\n                      cartilage and in the extracellular matrix of moderately osteoarthritic cartilage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitations<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\n                      was based on polyclonal antisera rather than a monoclonal antibody. There is a sex and breed bias within the groups of horses, also it could have been of value to include horses with septic arthritis and tendonitis and investigated joint differences.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This\n                      <jats:styled-content style=\"fixed-case\">COMP<\/jats:styled-content>\n                      neo\u2010epitope can be measured in sera, and results indicate that it could be a biomarker for pathologic fragmentation of cartilage in connection with acute joint lameness.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.13082","type":"journal-article","created":{"date-parts":[[2019,2,10]],"date-time":"2019-02-10T05:04:44Z","timestamp":1549775084000},"page":"674-680","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of circadian rhythm, age, training and acute lameness on serum concentrations of cartilage oligomeric matrix protein (\n                    <scp>COMP<\/scp>\n                    ) neo\u2010epitope in horses"],"prefix":"10.1111","volume":"51","author":[{"given":"S.","family":"Ekman","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Lindahl","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Transfusion Medicine Institute of Biomedicine Sahlgrenska University Hospital Gothenburg University Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"R\u00fcetschi","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Transfusion Medicine Institute of Biomedicine Sahlgrenska University Hospital Gothenburg University Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Jansson","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Biochemistry Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Bj\u00f6rkman","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Abrahamsson\u2010Aurell","sequence":"additional","affiliation":[{"name":"Halland Animal Hospital Kungsbacka Horse Clinic Kungsbacka Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Bj\u00f6rnsd\u00f3ttir","sequence":"additional","affiliation":[{"name":"Agricultural University of Iceland Hvanneyri, Borgarnes, Saudarkrokur Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"L\u00f6fgren","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Veterinary Public Health Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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