{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:23:49Z","timestamp":1792787029386,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,13]],"date-time":"2019-04-13T00:00:00Z","timestamp":1555113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Global Stem cell Technology NV","award":["130543"],"award-info":[{"award-number":["130543"]}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>There is a need to improve therapies for osteoarthritis in horses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To assess the efficacy of equine allogeneic chondrogenic\u2010induced mesenchymal stem cells combined with equine allogeneic plasma as a novel therapy for osteoarthritis in horses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Randomised, double\u2010blinded, placebo\u2010controlled experiment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In 12 healthy horses, osteoarthritis was induced in the metacarpophalangeal joint using an osteochondral fragment\u2010groove model. Five weeks after surgery, horses were randomly assigned to either an intra\u2010articular injection with chondrogenic\u2010induced mesenchymal stem cells + equine allogeneic plasma (= intervention) or with 0.9% saline solution (= control). From surgery until the study end, horses underwent a weekly joint and lameness assessment. Synovial fluid was collected for cytology and biomarker analysis before surgery and at Weeks 5, 5 + 1d, 7, 9 and 11. At Week 11, horses were subjected to euthanasia, and the metacarpophalangeal joints were evaluated macroscopically and histologically.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>No serious adverse events or suspected adverse drug reactions occurred during the study. A significant improvement in visual and objective lameness was seen with the intervention compared with the control. Synovial fluid displayed a significantly higher viscosity and a significantly lower glycosaminoglycan concentration in the intervention group. Other biomarkers or cytology parameters were not significantly different between the treatment groups. Significantly less wear lines and synovial hyperaemia were present in the intervention group. The amount of cartilage oligomeric matrix protein, collagen type II and glycosaminoglycans were significantly higher in the articular cartilage of the intervention group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitations<\/jats:title>\n                    <jats:p>This study assessed the short\u2010term effect of the intervention on a limited number of horses, using an osteoarthritis model. This study also included multiple statistical tests, increasing the risk of type 1 error.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Equine allogeneic chondrogenic\u2010induced mesenchymal stem cells combined with equine allogeneic plasma may be a promising treatment for osteoarthritis in horses.<\/jats:p>\n                    <jats:p>\n                      <jats:bold>The Summary is available in Spanish \u2013 see Supporting Information<\/jats:bold>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.13089","type":"journal-article","created":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T01:30:13Z","timestamp":1551317413000},"page":"787-794","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["The use of equine chondrogenic\u2010induced mesenchymal stem cells as a treatment for osteoarthritis: A randomised, double\u2010blinded, placebo\u2010controlled proof\u2010of\u2010concept study"],"prefix":"10.1111","volume":"51","author":[{"given":"S. Y.","family":"Broeckx","sequence":"first","affiliation":[{"name":"Global Stem Cell Technology NV Anacura Group Evergem Belgium"},{"name":"Department of Surgery and Anaesthesiology of Domestic Animals Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. M.","family":"Martens","sequence":"additional","affiliation":[{"name":"Department of Surgery and Anaesthesiology of Domestic Animals Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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