{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T12:30:50Z","timestamp":1779366650418,"version":"3.53.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T00:00:00Z","timestamp":1571875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Interleukin\u20106 (IL\u20106) is consistently increased in the digital lamellae in different studies of sepsis\u2010related laminitis (SRL). IL\u20106 signalling through the gp130 receptor activates similar signalling (i.e. mTORC1\u2010related signalling) previously reported to be activated in models of endocrinopathic laminitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To assess the activation state of signalling proteins downstream of IL\u20106\/gp130 receptor complex activation in an experimental model of SRL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Randomised experimental study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Lamellar phospho\u2010(P) protein concentrations downstream of the IL\u20106\/gp130 receptors were assessed in the oligofructose (OF) model of SRL. Fifteen Standardbred horses were administered water (CON, n\u00a0=\u00a08) or oligofructose (OF, n\u00a0=\u00a07) via a nasogastric tube. At 12\u00a0h post\u2010OF\/water administration, one randomly assigned forelimb was exposed to continuous digital hypothermia (CDH) by placement in ice water (ICE, maintained at &lt;7\u00b0C); the other forelimb was maintained at ambient temperature (AMB). Lamellar tissue samples were collected after 24\u00a0h of CDH from both ICE and AMB forelimbs and immediately snap\u2010frozen. Lamellar proteins of interest were assessed by immunoblotting and immunofluorescence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Immunoblotting revealed increase (P&lt;0.05) in the phosphorylation states of Akt (Ser 473), RPS6 (Ser235\/236), RPS6 (Ser240\/244), STAT3 (Ser727) and STAT3 (Tyr705) in lamellar tissue from OF\u2010treated animals (AMB OF vs. AMB CON limbs); CDH resulted in decreased (P&lt;0.05) lamellar concentrations of phosphorylated Akt, p70S6K, RPS6 (235\/236), RPS6 (240\/244) and STAT3 (S727) in OF\u2010treated animals (AMB OF vs. ICE OF). Immunofluorescence showed that activated\/phosphorylated forms of RPS6 and STAT3 were primarily localised to lamellar epithelial cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitations<\/jats:title>\n                    <jats:p>The nature, sequence and timing of sub\u2010cellular events in this experimental model may differ from those that accompany naturally occurring sepsis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>There were increased lamellar concentrations of activated signalling proteins downstream of the IL\u20106\/Gp130 receptor complex in OF\u2010treated horses; CDH inhibited this activation for the majority of the proteins assessed. These results demonstrate similar lamellar signalling (e.g. mTORC1\u2010related signalling) and, therefore, possible therapeutic targets occurring in sepsis\u2010related laminitis as previously reported in models of endocrinopathic laminitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.13184","type":"journal-article","created":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T11:20:40Z","timestamp":1568200840000},"page":"441-448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Influence of digital hypothermia on lamellar events related to IL\u20106\/gp130 signalling in equine sepsis\u2010related laminitis"],"prefix":"10.1111","volume":"52","author":[{"given":"K.","family":"Dern","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine Ohio State University Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9716-3452","authenticated-orcid":false,"given":"T. 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