{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:44:46Z","timestamp":1772160286143,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T00:00:00Z","timestamp":1603756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>As myosin heavy chain (MyHC) profile of muscle fibres is heavily influenced by neural input, changes in MyHC expression are expected in horses clinically affected with recurrent laryngeal neuropathy (RLN) yet, this has not been thoroughly investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To describe the changes in MyHC and fibre diameter in left cricoarytenoideus dorsalis (L\u2010CAD) muscle of horses with clinical signs of RLN.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Observational cohort study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immunohistochemistry was used to assess the MyHC\u2010based fibre\u2010type proportion, size and grouping in the L\u2010CAD of 10 Thoroughbred horses, five clinically affected with RLN and five unaffected controls based on resting endoscopic examination. The Mann\u2010Whitney U test was used to compare the two groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Compared to controls (of mean age 3.0\u00a0\u00b1\u00a01.7\u00a0years) which only expressed type I, IIA and IIX MyHC, the L\u2010CAD of affected horses (of mean age 2.8\u00a0\u00b1\u00a00.8\u00a0years) had obvious fibre\u2010type grouping, and despite apparent compensatory hypertrophy of a small number of fibres, a decrease in overall fibre diameter (median difference \u221235.2\u00a0\u00b5m, 95% CI \u221247.4 to \u22127.9,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.02) and diameter of type IIA fibres (median difference \u221246.8\u00a0\u00b5m, 95% CI \u221252.1 to \u22125.0,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.03) was observed. Anti\u2010fast MyHC (MY32) cross\u2010immunoreacted with embryonic\u2010MyHC. Whereas MY32\u2010positive fibres were identified as type IIX in controls, in affected horses these fibres were less than 50\u00a0\u00b5m diameter with internal nuclei and were MYH3\u2010positive for embryonic myosin indicating depletion of type IIX fibres, yet active regeneration and fibre renewal.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitations<\/jats:title>\n                    <jats:p>Small sample size that did not include subclinical cases. Fibre size and appearance rather than staining colour were relied upon to differentiate embryonic from type IIX MyHC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Horses clinically affected with RLN have overall atrophy of fibres, loss of IIX fibres and expression of embryonic myosin indicating regenerative capacity. Despite hypertrophy of some remaining fibres, the overall decline in the bulk of fibres, including those most fatigue\u2010resistant, may be the critical change that results in failure to maintain arytenoid abduction during exercise although direct comparison to subclinical cases is needed to confirm this.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.13362","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T12:28:04Z","timestamp":1601555284000},"page":"710-717","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immunohistochemical analysis of laryngeal muscle of horses clinically affected with recurrent laryngeal neuropathy"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7106-7884","authenticated-orcid":false,"given":"Catherine M.","family":"Steel","sequence":"first","affiliation":[{"name":"School of Veterinary and Agricultural Sciences University of Melbourne Werribee VIC Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Walmsley","sequence":"additional","affiliation":[{"name":"School of Veterinary and Agricultural Sciences University of Melbourne Werribee VIC Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Garry A.","family":"Anderson","sequence":"additional","affiliation":[{"name":"School of Veterinary and Agricultural Sciences University of Melbourne Werribee VIC Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantal A.","family":"Coles","sequence":"additional","affiliation":[{"name":"Murdoch Children's Research Institute The Royal Children's Hospital Parkville VIC Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5527-8964","authenticated-orcid":false,"given":"Benjamin","family":"Ahern","sequence":"additional","affiliation":[{"name":"School of Veterinary Science Equine Specialist Hospital University of Queensland Gatton QLD Australia"},{"name":"School of Animal and Veterinary Science The University of Adelaide Roseworthy Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason D.","family":"White","sequence":"additional","affiliation":[{"name":"Office of the Pro Vice Chancellor Research and Innovation Charles Sturt University Wagga Wagga NSW 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