{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:44:46Z","timestamp":1772160286761,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T00:00:00Z","timestamp":1606953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The current gold standard diagnostic test for leptospirosis is the microscopic agglutination test (MAT), which has many drawbacks; therefore, the development of a better and easier serological test for leptospirosis is needed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To apply reverse vaccinology (RV) and antigenic selection on the assortment of leptospiral targets and evaluate their potential for use as reagents for the diagnosis of equine leptospirosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Cross\u2010sectional study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The antigenic selection parameters were: proteins with antigenicity score \u22650.5 (VaxiJen), at least one B cell epitope and size between 10 and 275\u00a0KDa. New leptospiral proteins were cloned, expressed and serologically screened against equine sera (n\u00a0=\u00a0128) on a single analysis and comparative combinations. Sensitivity (Se) and specificity (Sp), accuracy, positive predictive value (PPV) and negative predictive value (NPV) were calculated. A BLAST with nucleotide and protein sequences was used to identify the serovar or species specificity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitations<\/jats:title>\n                    <jats:p>This cross\u2010sectional analysis had three main limitations: (a) The equine sera used in these tests were limited to sera submitted to the Animal Health Diagnosis Center and were only tested against seven serovars; (b) MAT results were considered being \u2018perfect\u2019, and the highest titre presented was considered being the infecting serovar, which may not hold true; (c) The strains used to represent the serovars and the limited number of different serovars and species included in the genetic analysis, which leads to the possibility that these proteins might be present in different species or serovars that perhaps would be seroprevalent in another geographic region.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The new leptospiral antigens described in this research could increase the sensitivity and specificity of ELISA for detection of\n                      <jats:italic>Leptospira<\/jats:italic>\n                      exposure and the detection of leptospirosis in horses along with support from other clinical signs. Some of these new antigens might be used to improve the detection of infecting serovar.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.13380","type":"journal-article","created":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T23:46:50Z","timestamp":1604274410000},"page":"1025-1035","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of new leptospiral antigens for the diagnosis of equine leptospirosis: An approach using pan\u2010genomic analysis, reverse vaccinology and antigenic selection"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1743-9664","authenticated-orcid":false,"given":"Tiago J.","family":"Zilch","sequence":"first","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences College of Veterinary Medicine Cornell University Ithaca New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jen\u2010Jie","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences College of Veterinary Medicine Cornell University Ithaca New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo C.","family":"Bressan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Vi\u00e7osa Vi\u00e7osa MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean P.","family":"McDonough","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences College of Veterinary Medicine Cornell University Ithaca New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hussni O.","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences College of Veterinary Medicine Cornell University Ithaca New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas J.","family":"Divers","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine Cornell University Ithaca New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8902-3089","authenticated-orcid":false,"given":"Yung\u2010Fu","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences College of Veterinary Medicine Cornell University Ithaca New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003898"},{"key":"e_1_2_11_3_1","first-page":"672","article-title":"Sero\u2010diagnostic de la spirochaetose icterohaemorrhagique","volume":"42","author":"Martin L","year":"1918","journal-title":"Bull Mem Soc Med Hop Paris"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/am.25.6.976-980.1973"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"e_1_2_11_6_1","unstructured":"Control C.f.D. and Prevention.Leptospirosis (Leptospira interrogans) 2013 Case Definition. 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