{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:44:50Z","timestamp":1772160290064,"version":"3.50.1"},"reference-count":75,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T00:00:00Z","timestamp":1655078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100015103","name":"Japan Racing Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015103","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Equine premature placental separation (PPS) is poorly understood and represents an important risk factor for fetal\/neonatal hypoxia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To examine transcriptomic changes in the chorioallantois (CA) from mares with clinical PPS compared with the CA from normal foaling mares. Differential gene expression was determined and gene ontology as well as molecular pathways related to PPS were characterised.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Retrospective case: control study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      CA were collected from Thoroughbred mares with a clinical history of PPS (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a033) and from control Thoroughbred mares (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a04) with normal parturition for examination of transcriptional changes in the placenta associated with PPS. Transcriptomic changes in the villous CA near the cervical star were determined by Illumina\u00ae sequencing and subsequent bioinformatic analysis. PPS samples were divided by k\u2010means clustering, and differentially expressed genes (DEGs) in each PPS cluster were identified by comparing to controls. Shared DEGs between PPS clusters were used for gene ontology analysis and pathway analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 1204 DEGs were identified between PPS and control. Gene ontology revealed extracellular matrix (ECM) and cell adhesion, and pathway analysis revealed fatty acid, p\u201053, hypoxia and inflammation. Eleven key regulator genes of PPS including growth factors (\n                      <jats:italic>IGF1<\/jats:italic>\n                      ,\n                      <jats:italic>TGFB2<\/jats:italic>\n                      ,\n                      <jats:italic>TGFB3<\/jats:italic>\n                      ), transcription factors (\n                      <jats:italic>HIF1A<\/jats:italic>\n                      ,\n                      <jats:italic>JUNB<\/jats:italic>\n                      ,\n                      <jats:italic>SMAD3<\/jats:italic>\n                      ), and transmembrane receptors (\n                      <jats:italic>FGFR1<\/jats:italic>\n                      ,\n                      <jats:italic>TNFRSF1A<\/jats:italic>\n                      ,\n                      <jats:italic>TYROBP<\/jats:italic>\n                      ) were also identified.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitations<\/jats:title>\n                    <jats:p>The use of clinical history of PPS, in the absence of other criteria, may have led to misidentification of some cases as PPS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Transcriptomic analysis indicated that changes in ECM and cell adhesion were important factors in equine PPS. Key predicted upstream events include genes associated with hypoxia, inflammation and growth factors related to the pathogenesis of equine PPS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.13602","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T11:18:31Z","timestamp":1653650311000},"page":"405-418","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transcriptomic analysis of the chorioallantois in equine premature placental separation"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6723-6283","authenticated-orcid":false,"given":"Harutaka","family":"Murase","sequence":"first","affiliation":[{"name":"Gluck Equine Research Center, Department of Veterinary Science University of Kentucky  Lexington Kentucky USA"},{"name":"Equine Science Division, Hidaka Training and Research Center Japan Racing Association  Hokkaido 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