{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T00:18:05Z","timestamp":1793405885411,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      is a protozoan parasite causing diarrhoea in humans and animals. Although\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      has been found in domestic horses (farmed or kept at pasture), there has been only one published study of\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      infections in Chinese racehorses, which was restricted to a very small geographical area.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To investigate the presence of\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      spp. in the faeces of racehorses in China and to perform molecular characterisation of the parasite.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Cross\u2010sectional.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 621 fresh faecal samples were collected for DNA extraction from racehorses at 17 equestrian clubs from 12 provinces of China from December 2016 to May 2018. All the DNA were analysed for the presence of\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      species\/genotypes and subtypes by PCR amplification of the small subunit ribosomal RNA and 60\u2009kDa glycoprotein genes respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      PCR analysis revealed that 11 samples (1.8%) were positive for\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      spp. Among them seven samples were identified as\n                      <jats:italic>C. parvum<\/jats:italic>\n                      and four were\n                      <jats:italic>C. hominis<\/jats:italic>\n                      . The\n                      <jats:italic>C. parvum<\/jats:italic>\n                      isolates were identified as subtype IIdA14G1 (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a04) and IIdA15G1 (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a03), while all\n                      <jats:italic>C. hominis<\/jats:italic>\n                      isolates were identified as subtype IkA18G1 (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a04).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitations<\/jats:title>\n                    <jats:p>A single faecal sample from each horse was used instead of multiple samples that could improve the detection rates of the parasite.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Although\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      infection rate was relatively low in the investigated racehorses, the presence of zoonotic subtypes IIdA14G and 1IIdA15G1 of\n                      <jats:italic>C. parvum<\/jats:italic>\n                      and IkA18G1 of\n                      <jats:italic>C. hominis<\/jats:italic>\n                      , suggesting that these animals are a potential source of\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      in humans.\n                    <\/jats:p>\n                  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