{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T18:05:26Z","timestamp":1788458726502,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T00:00:00Z","timestamp":1673222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Bacteria belonging to the genus\n                      <jats:italic>Mycoplasma<\/jats:italic>\n                      are small\u2010sized, have no cell walls and small genomes. They commonly cause respiratory disorders in their animal hosts. Three species have been found in the respiratory tract of horses worldwide, that is.,\n                      <jats:italic>Mycoplasma<\/jats:italic>\n                      (\n                      <jats:italic>M.<\/jats:italic>\n                      )\n                      <jats:italic>equirhinis<\/jats:italic>\n                      ,\n                      <jats:italic>M. pulmonis<\/jats:italic>\n                      and\n                      <jats:italic>M. felis<\/jats:italic>\n                      , but their role in clinical cases remains unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      The aim of this study was to i) develop and validate tools to detect, isolate and identify different\n                      <jats:italic>Mycoplasma<\/jats:italic>\n                      spp. strains in clinical equine respiratory\u2010tract specimens and ii) subsequently define the prevalence of the three species in France depending on sample types and horse characteristics (age, breed, sex).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Validation of a workflow for mycoplasma diagnosis and subsequent prevalence study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mycoplasma\u2010free tracheal wash samples spiked with numerated strains and DNA dilutions were used to validate the culture methods and real\u2010time PCR (rt\u2010PCR) assay. Isolated strains were identified by 16S rRNA gene sequencing. Prevalences were determined on a population of 616 horses with respiratory disorders, sampled in France in 2020.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In total, 104 horses (16.9%) were found to be positive for\n                      <jats:italic>Mycoplasma<\/jats:italic>\n                      spp. by at least one method.\n                      <jats:italic>M. equirhinis<\/jats:italic>\n                      was the predominant circulating species, accounting for 85% of the rt\u2010PCR\u2010positive samples and 98% of the 40 cultured strains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitation<\/jats:title>\n                    <jats:p>The proposed pre\u2010enrichment procedure improves the sensitivity of detection but hinders the quantification of the initial mycoplasma load in the clinical specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Prevalence of mycoplasma varied with age, breed, and type of sample.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.13918","type":"journal-article","created":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T23:16:47Z","timestamp":1672096607000},"page":"747-754","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Detection of\n                    <i>Mycoplasma<\/i>\n                    spp. in horses with respiratory disorders"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9143-9072","authenticated-orcid":false,"given":"Matthieu","family":"Martineau","sequence":"first","affiliation":[{"name":"LAB\u00c9O, Research Department, St Contest  Caen France"},{"name":"NormandieUniversit\u00e9 CAEN\/ROUEN Universit\u00e9s, DYNAMICURE, INSERM U1311  France"},{"name":"Universit\u00e9 de Lyon Anses, VetAgro Sup, UMR Mycoplasmoses Animales  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Castagnet","sequence":"additional","affiliation":[{"name":"LAB\u00c9O, Research Department, St Contest  Caen France"},{"name":"NormandieUniversit\u00e9 CAEN\/ROUEN Universit\u00e9s, DYNAMICURE, INSERM U1311  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Kokabi","sequence":"additional","affiliation":[{"name":"LAB\u00c9O, Research Department, St Contest  Caen France"},{"name":"NormandieUniversit\u00e9 CAEN\/ROUEN Universit\u00e9s, DYNAMICURE, INSERM U1311  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agn\u00e8s","family":"Tricot","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon Anses, VetAgro Sup, UMR Mycoplasmoses Animales  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0443-5565","authenticated-orcid":false,"given":"Maryne","family":"Ja\u00ff","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon Anses, VetAgro Sup, UMR Mycoplasmoses Animales  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3151-4947","authenticated-orcid":false,"given":"Albertine","family":"L\u00e9on","sequence":"additional","affiliation":[{"name":"LAB\u00c9O, Research Department, St Contest  Caen France"},{"name":"NormandieUniversit\u00e9 CAEN\/ROUEN Universit\u00e9s, DYNAMICURE, INSERM U1311  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3968-4801","authenticated-orcid":false,"given":"Florence","family":"Tardy","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon Anses, VetAgro Sup, UMR Mycoplasmoses Animales  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.4.1094-1156.1998"},{"key":"e_1_2_11_3_1","volume-title":"Methods in Mycoplasmology. 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