{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:54:05Z","timestamp":1792025645768,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/13883\u20109"],"award-info":[{"award-number":["2017\/13883\u20109"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2024,7]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Artificial insemination with cooled\u2010shipped semen is the primary method used in the equine breeding industry; yet, sperm quality and fertility can be suboptimal for some stallions when standard techniques are used. Therefore, there is a critical need to develop alternative approaches for these stallions.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n To assess sperm quality parameters and fertility of cooled\u2010stored stallion semen processed by SpermFilter\u00ae or centrifugation and resuspended in three extenders.<\/jats:p>\n <\/jats:sec>\n \n Study design<\/jats:title>\n Controlled and field study.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In Experiment 1, semen was collected from 21 stallions classified as having good (\u2018\n Good\u2010coolers<\/jats:italic>\n \u2019,\n n<\/jats:italic>\n \u2009=\u20098) or poor (\u2018\n Bad\u2010coolers<\/jats:italic>\n \u2019,\n n<\/jats:italic>\n \u2009=\u200913) semen cooling. The semen was extended at 30 million spermatozoa\/mL in a skimmed milk\u2010based (SM) diluent, and refrigerated for 24\u2009h. Then, the cooled\u2010stored semen was processed through SpermFilter\u00ae or centrifugation, and the resulting sperm pellets were resuspended in SM, SM containing pentoxifylline (SM\u2010P), or an egg yolk\u2010based (EY) extender. Unprocessed cooled\u2010stored semen served as control. Sperm motility parameters, plasma membrane integrity (PMI), and mitochondrial membrane potential (HMMP) were assessed in cooled\u2010semen pre\u2010 and post\u2010processing. Experiment 2, cooled semen from 9 stallions classified as\n Bad\u2010cooler<\/jats:italic>\n s was used to inseminate 18 embryo donor mares at 66\u2009cycles (Unprocessed,\n n<\/jats:italic>\n \u2009=\u200922; SpermFilter\u00ae\/SM\u2010P,\n n<\/jats:italic>\n \u2009=\u200916; or SpermFilter\u00ae\/EY,\n n<\/jats:italic>\n \u2009=\u200928). Data were analysed with a mixed model and Tukey's as posthoc, and logistic regression.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Processed semen resuspended in EY had superior sperm motility compared to unprocessed, SM and SM\u2010P (\n p<\/jats:italic>\n \u2009<\u20090.0001). Semen processed by SpermFilter\u00ae resuspended in SM\u2010P was similar to EY (\n p<\/jats:italic>\n \u2009>\u20090.05). Pellet resuspension with EY and SM\u2010P improved the HMMP of\n Bad\u2010cooler<\/jats:italic>\n stallions (\n p<\/jats:italic>\n \u2009=\u20090.0010). Semen processed by SpermFilter\u00ae had superior PMI to centrifuged semen (\n p<\/jats:italic>\n \u2009<\u20090.0001). Mares inseminated with SpermFilter\u00ae\/SM\u2010P (50%, 8\/16) or SpermFilter\u00ae\/\u2010EY (68%, 9\/28) had higher pregnancy rates than mares bred with unprocessed semen (14%, 3\/22) (\n p<\/jats:italic>\n \u2009<\u20090.001).\n <\/jats:p>\n <\/jats:sec>\n \n Main limitations<\/jats:title>\n Low number of mares in the fertility trial.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Sperm quality and fertility of\n Bad\u2010cooler<\/jats:italic>\n stallions can be enhanced by SpermFilter\u00ae and pellet resuspension with either EY or SM\u2010P.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/evj.14085","type":"journal-article","created":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T04:05:51Z","timestamp":1712117151000},"page":"697-710","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Post\u2010cooling semen processing and sperm re\u2010suspension as an alternative method to circumvent poor semen cooling in stallions"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7526-7760","authenticated-orcid":false,"given":"Lorenzo","family":"Segabinazzi","sequence":"first","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, FMVZ Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d, UNESP Botucatu Brazil"},{"name":"Ross University School of Veterinary Medicine Basseterre St. Kitts West Indies"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcio T.","family":"Carmo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, FMVZ Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d, UNESP Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila de P.","family":"Freitas\u2010Dell'Aqua","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, FMVZ Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d, UNESP Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco A.","family":"Alvarenga","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, FMVZ Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d, UNESP Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederico O.","family":"Papa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, FMVZ Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d, UNESP Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3799-6641","authenticated-orcid":false,"given":"Igor F.","family":"Canisso","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Medicine College of Veterinary Medicine, University of Illinois Urbana\u2010Champaign Urbana Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,3]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-52-40"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.10.027"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.04.015"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00719.x"},{"key":"e_1_2_13_6_1","first-page":"1323","volume-title":"Equine reproduction","author":"Robinson 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