{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:09:06Z","timestamp":1787238546195,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["251133687\/GRP2046"],"award-info":[{"award-number":["251133687\/GRP2046"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2025,3]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Surveillance of\n Strongylus vulgaris<\/jats:italic>\n and other\n Strongylus<\/jats:italic>\n spp. in equids is important for targeted intervention in parasite control, requiring reliable routine diagnostic methods.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n Comparing morphological examination and PCR analyses of larval cultures to identify\n Strongylus<\/jats:italic>\n spp. species based on German diagnostic samples from 2018.\n <\/jats:p>\n <\/jats:sec>\n \n Study design<\/jats:title>\n Method comparison.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n During the routine diagnostic investigations, in total 712 strongyle\u2010egg positive equine faecal samples were cultured. Third\u2010stage larvae (L3) were morphologically differentiated. For molecular validation, samples were examined using\n S. vulgaris<\/jats:italic>\n real\u2010time PCR and\n Strongylus edentatus<\/jats:italic>\n \/\n Strongylus equinus<\/jats:italic>\n \/\n Strongylus asini<\/jats:italic>\n high\u2010resolution melting PCRs.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Based on 28S rRNA PCR, 594 samples positive for nematode DNA were included in the study. The inter\u2010rater reliability to compare morphological and molecular species identification was fair for\n Strongylus<\/jats:italic>\n spp. without species identification and for\n S. edentatus<\/jats:italic>\n , slight for\n S. equinus<\/jats:italic>\n and poor for\n S. vulgaris<\/jats:italic>\n . The frequency based on morphological and molecular data in this study were for\n S. vulgaris<\/jats:italic>\n 0% and 0.8%, respectively, for\n S. edentatus<\/jats:italic>\n 0.3% and 1.5%, respectively, and for\n S. equinus<\/jats:italic>\n 2.0% and 0.2%, respectively. Based on molecular analyses, one sample obtained from a domestic horse contained\n S. asini<\/jats:italic>\n DNA, which was confirmed by sequencing.\n <\/jats:p>\n <\/jats:sec>\n \n Main limitations<\/jats:title>\n For many samples, no or only incomplete data regarding clinical history, the exact geographical location and whether samples were obtained on individual or farm level, were available.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Results of morphological and molecular examination methods of strongyle L3 from equine samples can differ substantially. Further evaluation of these methods is required to provide reliable and cost\u2010effective methods of screening equine parasites. Further studies using approaches suitable to detect\n S. asini<\/jats:italic>\n are needed to evaluate its clinical and epidemiological relevance.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/evj.14134","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T08:30:16Z","timestamp":1721118616000},"page":"522-529","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Comparison of morphological and molecular\n Strongylus<\/i>\n spp. identification in equine larval cultures and first report of a patent\n Strongylus asini<\/i>\n infection in a horse"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6637-7490","authenticated-orcid":false,"given":"Irina","family":"Diekmann","sequence":"first","affiliation":[{"name":"Institute for Parasitology and Tropical Veterinary Medicine, Freie Universit\u00e4t Berlin Berlin Germany"},{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Blazejak","sequence":"additional","affiliation":[{"name":"Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2842-8100","authenticated-orcid":false,"given":"J\u00fcrgen","family":"Kr\u00fccken","sequence":"additional","affiliation":[{"name":"Institute for Parasitology and Tropical Veterinary Medicine, Freie Universit\u00e4t Berlin Berlin Germany"},{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9093-1006","authenticated-orcid":false,"given":"Christina","family":"Strube","sequence":"additional","affiliation":[{"name":"Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6043-2038","authenticated-orcid":false,"given":"Georg","family":"von Samson\u2010Himmelstjerna","sequence":"additional","affiliation":[{"name":"Institute for Parasitology and Tropical Veterinary Medicine, Freie Universit\u00e4t Berlin Berlin Germany"},{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin Berlin 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