{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T10:04:06Z","timestamp":1789639446095,"version":"4.0.0"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T00:00:00Z","timestamp":1725408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>It is unknown whether short\u2010term transportation affects endocrine responses similarly in horses with and without insulin dysregulation (ID).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To characterise the effect of short\u2010term transportation on stress parameters and insulin responses to an oral sugar test (OST) in horses with and without ID.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Longitudinal cohort study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Fourteen adult non\u2010pregnant, non\u2010PPID mares of mixed light breeds were grouped as either ID (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20097) or non\u2010ID (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20097) based on endocrine testing. Over 2\u2009weeks, horses were transported once, in groups of 3\u20134 in a horse trailer on a round\u2010trip journey of ~1.5\u2009h. Blood and saliva were collected 24\u2009h and 1\u2009h pre\u2010transportation, directly after unloading and 15\u2009min, 1\u2009h, 3\u2009h plus 24\u2009h post\u2010transportation. An OST was performed 24\u2009h pre\u2010transportation and 3\u2009h post\u2010transportation with a pre\u2010 (T0) and post\u2010OST sample collected 60\u2009min later (T60). Heart rates and rectal temperatures were also collected throughout the study. Serum insulin, serum cortisol, and plasma glucose were measured using validated assays. Repeated measures ANOVA were used to determine differences after transportation and between ID and non\u2010ID horses. Non\u2010normal data were log\u2010transformed and multiple comparisons were adjusted using Bonferroni post hoc tests.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Mean insulin was higher in ID horses versus non\u2010ID horses (mean\u2009=\u2009109.9 \u03bcU\/mL vs. 30.2 \u03bcU\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001; 95% CI for mean difference\u2009=\u2009[55.6\u2013107.7 \u03bcU\/mL]). Mean serum insulin increased following OST at T60 in ID horses pre\u2010 (154.6 \u03bcU\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.04; 95% CI\u2009=\u2009[86.3\u2013223.0 \u03bcU\/mL]) and post\u2010transportation (284.6 \u03bcU\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.03; 95% CI\u2009=\u2009[114.3\u2013454.8 \u03bcU\/mL]). Non\u2010ID horses had a mean OST T60 insulin post\u2010transportation of 56.6 \u03bcU\/mL (95% CI\u2009=\u2009[29.1\u201384.1 \u03bcU\/mL]); above recognised threshold [45 \u03bcU\/mL] for ID diagnosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main limitations<\/jats:title>\n                    <jats:p>Small number of horses, only mares used, and OST not performed immediately post\u2010transportation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Performing an OST 3\u2009h following short\u2010term transportation may result in inaccurate ID status.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/evj.14403","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T01:05:13Z","timestamp":1725498313000},"page":"745-755","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The impact of short\u2010term transportation stress on insulin and oral sugar responses in insulin dysregulated and non\u2010insulin dysregulated horses"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3062-2647","authenticated-orcid":false,"given":"Erica T.","family":"Jacquay","sequence":"first","affiliation":[{"name":"M. H. Gluck Equine Research Center, Department of Veterinary Science University of Kentucky  Lexington Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Harris","sequence":"additional","affiliation":[{"name":"Equine Studies Group Waltham Petcare Science Institute, Waltham on the Wolds  Leicestershire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda A.","family":"Adams","sequence":"additional","affiliation":[{"name":"M. H. Gluck Equine Research Center, Department of Veterinary Science University of Kentucky  Lexington Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,4]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.12169"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2010.0503.x"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2016.01.013"},{"key":"e_1_2_14_5_1","first-page":"1","volume-title":"Recommendations for the diagnosis and treatment of equine metabolic syndrome (EMS)","author":"Frank N","year":"2022"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2021.103770"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jveb.2011.04.007"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.13110"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.2527\/2000.7861458x"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/09712119.2015.1091340"},{"key":"e_1_2_14_11_1","first-page":"112","volume-title":"7th European workshop on equine nutrition","author":"Pratt\u2010Phillips S","year":"2014"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2023.104879"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2023.104402"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.14019"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1983.tb01826.x"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2008.02.029"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11072051"},{"key":"e_1_2_14_18_1","volume-title":"Recommendations on diagnosis and management of pituitary pars intermedia dysfunction (PPID)","author":"McFarlane D","year":"2023"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0254139"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.13474"},{"key":"e_1_2_14_21_1","volume-title":"Nutrient requirements of horses: sixth revised edition","author":"National Research Council","year":"2007"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jveb.2014.12.002"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2020.106448"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2016.04.002"},{"key":"e_1_2_14_25_1","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2021.626427","article-title":"The interaction of insulin and pituitary hormone syndromes","volume":"12","author":"Schernthaner\u2010Reiter MH","year":"2021","journal-title":"Front Endocrinol (Lausanne)"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2895-8_5"},{"key":"e_1_2_14_27_1","first-page":"940","article-title":"Effects of pretreatment with dexamethasone or levothyroxine sodium on endotoxin\u2010induced alterations in glucose and insulin dynamics in horses","volume":"61","author":"Toth F","year":"2010","journal-title":"J Vet Intern Med"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.74.7.1010"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.12963"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9111204"},{"key":"e_1_2_14_31_1","first-page":"623","article-title":"Salivary and plasma insulin levels in man","volume":"30","author":"Fekete Z","year":"1993","journal-title":"Biochem Mol Biol Int"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1530\/EC-12-0024"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2008.10.026"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2009.11.003"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.13839"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2020.106531"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2010.06.002"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.applanim.2008.08.013"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.162.19.609"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas2017.1670"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2019.00027"},{"key":"e_1_2_14_42_1","doi-asserted-by":"publisher","DOI":"10.1294\/jes.15.99"},{"key":"e_1_2_14_43_1","doi-asserted-by":"publisher","DOI":"10.1207\/s15327604jaws0604_1"},{"key":"e_1_2_14_44_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.73.4.515"},{"key":"e_1_2_14_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.939534"},{"key":"e_1_2_14_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jveb.2011.09.003"},{"key":"e_1_2_14_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2017.01.024"},{"key":"e_1_2_14_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23073636"},{"key":"e_1_2_14_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.07.002"},{"key":"e_1_2_14_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13020211"},{"key":"e_1_2_14_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.14725"}],"container-title":["Equine Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/evj.14403","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T08:53:52Z","timestamp":1762073632000},"score":1,"resource":{"primary":{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/10.1111\/evj.14403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,4]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1111\/evj.14403"],"URL":"https:\/\/doi.org\/10.1111\/evj.14403","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/EVJ.14403\/v2\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EVJ.14403\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EVJ.14403\/v1\/review4","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EVJ.14403\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EVJ.14403\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EVJ.14403\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EVJ.14403\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EVJ.14403\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EVJ.14403\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EVJ.14403\/v2\/response1","asserted-by":"object"}]},"ISSN":["0425-1644","2042-3306"],"issn-type":[{"value":"0425-1644","type":"print"},{"value":"2042-3306","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,4]]},"assertion":[{"value":"2024-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}