{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T06:47:57Z","timestamp":1700549277747},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,10,15]],"date-time":"2012-10-15T00:00:00Z","timestamp":1350259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Spontaneous melanoma models in transgenic mice are increasingly used in preclinical research as they most closely match the progression of melanoma in humans. While optical inspection only allows analysis of tumors located on the skin, the accurate measurement and growth of subcutaneous tumors have not been adequately assessed. To improve the measurement accuracy of melanoma tumors, we used a fast single\u2010sequence <jats:styled-content style=\"fixed-case\">MRI<\/jats:styled-content> protocol at 9.4 Tesla for longitudinal characterization of a <jats:italic>ret<\/jats:italic>\u2010transgenic mouse model. Repeated <jats:styled-content style=\"fixed-case\">MRI<\/jats:styled-content> (average acquisition time 30\u00a0min per animal) of the trunk (excluding head and distal limbs) in six siblings revealed an increase in the mean total tumor volume (<jats:styled-content style=\"fixed-case\">TTV<\/jats:styled-content>) from 102.0\u00a0\u00b1\u00a080.5\u00a0mm<jats:sup>3<\/jats:sup> at 35\u00a0days of age to 434.8\u00a0\u00b1\u00a0154.9\u00a0mm<jats:sup>3<\/jats:sup> by 77\u00a0days. The main tumor load was located within the pelvis (&gt;40%), followed by the proximal hind limbs and groins (&gt;30%). The smallest detectable tumor measured 0.07\u00a0mm<jats:sup>3<\/jats:sup>. Inter\u2010rater reliability between a radiologist and a veterinarian analysing <jats:styled-content style=\"fixed-case\">MRI<\/jats:styled-content> data was 0.993 for <jats:styled-content style=\"fixed-case\">TTV<\/jats:styled-content> and 0.840 for number of tumors (both p\u00a0&lt;\u00a00.001). We thus conclude that because of the high variance of <jats:styled-content style=\"fixed-case\">TTV<\/jats:styled-content> of same\u2010aged mice, <jats:styled-content style=\"fixed-case\">MRI<\/jats:styled-content> should be used (i) to establish treatment groups matched for <jats:styled-content style=\"fixed-case\">TTV<\/jats:styled-content> and (ii) for longitudinal examination of the <jats:styled-content style=\"fixed-case\">TTV<\/jats:styled-content> in mice over the course of treatments.<\/jats:p>","DOI":"10.1111\/exd.12013","type":"journal-article","created":{"date-parts":[[2012,8,21]],"date-time":"2012-08-21T11:21:06Z","timestamp":1345548066000},"page":"837-841","source":"Crossref","is-referenced-by-count":0,"title":["Characterization and longitudinal monitoring of melanoma growth in <i>ret<\/i>\u2010transgenic mice using a single\u2010sequence <scp>MRI<\/scp> protocol"],"prefix":"10.1111","volume":"21","author":[{"given":"Hans U.","family":"Kerl","sequence":"first","affiliation":[{"name":"Department of Neuroradiology Medical Faculty Mannheim University of Heidelberg Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanne","family":"Boll","sequence":"additional","affiliation":[{"name":"Department of Neuroradiology Medical Faculty Mannheim University of Heidelberg Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Ramacher","sequence":"additional","affiliation":[{"name":"Skin Cancer Unit, German Cancer Research Center Heidelberg and Department of Dermatology, Venereology and Allergology University Medical Center Mannheim, Ruprecht\u2010Karl University of Heidelberg Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Heilmann","sequence":"additional","affiliation":[{"name":"Medical Faculty Mannheim Computer Assisted Clinical Medicine University of Heidelberg Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Groden","sequence":"additional","affiliation":[{"name":"Department of Neuroradiology Medical Faculty Mannheim University of Heidelberg Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Kramer","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences Small Animal Clinic Justus\u2010Liebig\u2010University Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktor","family":"Umansky","sequence":"additional","affiliation":[{"name":"Skin Cancer Unit, German Cancer Research Center Heidelberg and Department of Dermatology, Venereology and Allergology University Medical Center Mannheim, Ruprecht\u2010Karl University of Heidelberg Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc 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