{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:53:38Z","timestamp":1789516418616,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,10,15]],"date-time":"2012-10-15T00:00:00Z","timestamp":1350259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In recent times, the role of <jats:styled-content style=\"fixed-case\">LXR<\/jats:styled-content>s in skin physiology and pathology has evolved rapidly because of their role in proliferation, carcinogenesis, differentiation and permeability barrier function. <jats:styled-content style=\"fixed-case\">LXR<\/jats:styled-content>s were identified as promising drug targets for the treatment of many skin diseases. For this study, skin biopsies were taken from 15 patients with vitiligo and six controls to culture melanocytes from clinically active perilesional and normal skin. Gene expression was examined by reverse transcriptase\u2013polymerase chain reaction analysis. Role of <jats:styled-content style=\"fixed-case\">LXR<\/jats:styled-content>\u2010\u03b1 in regulating the expression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s was checked by gene knock\u2010down, and its role in vitiligo pathogenesis was checked by treatment with <jats:styled-content style=\"fixed-case\">LXR<\/jats:styled-content>\u2010\u03b1 agonist 22(<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>)\u2010hydroxycholesterol. After treatment adhesion assay, annexin <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content> staining and proliferation assay were performed. The expression of <jats:styled-content style=\"fixed-case\">LXR<\/jats:styled-content>\u2010\u03b1 was relatively more in perilesional skin melanocytes as compared to uninvolved skin melanocytes of non\u2010segmental vitiligo patient, and controls on the other hand, perilesional melanocytes were more prone to apoptosis. <jats:styled-content style=\"fixed-case\">LXR<\/jats:styled-content>\u2010\u03b1 gene knock\u2010down significantly increases the expression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">LXR<\/jats:styled-content>\u2010\u03b1 agonist 22(<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>)\u2010hydroxycholesterol treatment significantly decreases melanocyte adhesion, apoptosis and proliferation. Higher expression of <jats:styled-content style=\"fixed-case\">LXR<\/jats:styled-content>\u2010\u03b1 in perilesional skin melanocytes significantly decreases the adhesion, proliferation and matrix metalloproteinases and increases apoptosis.<\/jats:p>","DOI":"10.1111\/exd.12017","type":"journal-article","created":{"date-parts":[[2012,8,24]],"date-time":"2012-08-24T06:15:08Z","timestamp":1345788908000},"page":"853-858","source":"Crossref","is-referenced-by-count":21,"title":["Altered levels of <scp>LXR<\/scp>\u2010\u03b1: crucial implications in the pathogenesis of vitiligo"],"prefix":"10.1111","volume":"21","author":[{"given":"Ravinder","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Dermatology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davinder","family":"Parsad","sequence":"additional","affiliation":[{"name":"Department of Dermatology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amrinderjit","family":"Kanwar","sequence":"additional","affiliation":[{"name":"Department of Dermatology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak","family":"Kaul","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine and Biotechnology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,10,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4362.1988.tb01260.x"},{"key":"e_1_2_8_3_1","first-page":"1","volume":"23","author":"Parsad D","year":"2003","journal-title":"Health Qual Life 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