{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T17:12:12Z","timestamp":1779124332277,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,11,22]],"date-time":"2012-11-22T00:00:00Z","timestamp":1353542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hair root harbours a complex cell pool with an immense developmental potential. Several lineages, including skin, can be differentiated from the multipotent to pluripotent cells of outer root sheath (<jats:styled-content style=\"fixed-case\">ORS<\/jats:styled-content>) of hair follicle. Outer root sheath presents the most opulent non\u2010invasively gained adult stem cell source known. For the purposes of cultivating melanocytes designated for graft\u2010based treatments of depigmentation disorders, we have established an <jats:italic>ex vivo\/in vitro<\/jats:italic> cultivation method by introducing several methodological improvements to the <jats:styled-content style=\"fixed-case\">ORS<\/jats:styled-content> explant method of <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>ieckmann. As a result, we gained a higher, purer yield of differentiated melanocytes in half the time (at least 10<jats:sup>6<\/jats:sup> of 95% pure cells in 4\u00a0weeks). This reliable cultivation procedure begins with the epilation of 60 hairs and yields high numbers of <jats:styled-content style=\"fixed-case\">ORS<\/jats:styled-content> melanocytes that could be used for grafting applications. The procedure not only utilises the developmental potential of hair root cell pool and favors differentiation into melanocytes, but also contributes to the general trend of minimal\u2010to\u2010non\u2010invasive strategies for regenerative medicine.<\/jats:p>","DOI":"10.1111\/exd.12038","type":"journal-article","created":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T07:07:35Z","timestamp":1349075255000},"page":"948-950","source":"Crossref","is-referenced-by-count":18,"title":["Improved method of differentiation, selection and amplification of human melanocytes from the hair follicle cell pool"],"prefix":"10.1111","volume":"21","author":[{"given":"Vuk","family":"Savkovic","sequence":"first","affiliation":[{"name":"Translational Centre for Regenerative Medicine (TRM) Leipzig University Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Dieckmann","sequence":"additional","affiliation":[{"name":"Human and Environmental Toxicology University of Konstanz Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Milkova","sequence":"additional","affiliation":[{"name":"Department of Dermatology Venerology and Allergology Leipzig University Medical Center Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan C.","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Dermatology Venerology and Allergology Leipzig University Medical Center Leipzig 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