{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:54:39Z","timestamp":1791356079833,"version":"4.3.1"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,11,22]],"date-time":"2012-11-22T00:00:00Z","timestamp":1353542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Systemic antipsoriatic therapies have potentially life\u2010threatening, long\u2010term side effects. The efficacy of topical drugs is poor, but may be improved by the use of delivery systems based on drug nanoparticles. To produce nanoparticles (NP) composed of cyclosporin A, a classical antipsoriatic drug, and to investigate their penetration and biological effects in human skin affected by psoriatic symptoms, poly\u2010\u03b5\u2010caprolactone (PCL) and cyclosporin A (CsA) NP were prepared by the solvent evaporation method. Skin penetration was followed using fluorescently labeled NP in human skin organ cultures (<jats:styled-content style=\"fixed-case\">hSOC<\/jats:styled-content>). Psoriatic symptoms were mimicked in <jats:styled-content style=\"fixed-case\">hSOC<\/jats:styled-content> by the treatment with epidermal growth factor (EGF) and bacterial lipopolysaccharide (LPS). Cell viability in <jats:styled-content style=\"fixed-case\">hSOC<\/jats:styled-content> was evaluated by the resazurin test, and cytokine secretion into the growth medium was measured by immunodetection. We showed that topically applied NP diffused throughout the epidermis within two hours and through the dermis within the following day. They significantly reduced the secretion of inflammatory cytokines IL\u20131\u03b2, IL\u20136, IL\u20138, IL\u201320 and IL\u201323. At active doses, no cytotoxicity was detected. This type of NP display relevant properties for the use as topical anti\u2010inflammatory agents and may help to resorb psoriatic lesions.<\/jats:p>","DOI":"10.1111\/exd.12051","type":"journal-article","created":{"date-parts":[[2012,10,17]],"date-time":"2012-10-17T11:51:11Z","timestamp":1350474671000},"page":"938-943","source":"Crossref","is-referenced-by-count":31,"title":["Penetration and biological effects of topically applied cyclosporin <scp>A<\/scp> nanoparticles in a human skin organ culture inflammatory model"],"prefix":"10.1111","volume":"21","author":[{"given":"Marina","family":"Fru\u0161i\u0107\u2010Zlotkin","sequence":"first","affiliation":[{"name":"Department of Biological Chemistry Silberman Institute of Life Sciences The Hebrew University of Jerusalem Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoram","family":"Soroka","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry Silberman Institute of Life Sciences The Hebrew University of Jerusalem Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Tivony","sequence":"additional","affiliation":[{"name":"Casali Center for Applied Chemistry Institute of Chemistry The Hebrew University of Jerusalem Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liraz","family":"Larush","sequence":"additional","affiliation":[{"name":"Casali Center for Applied Chemistry Institute of Chemistry The Hebrew University of Jerusalem Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilian","family":"Verkhovsky","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry Silberman Institute of Life Sciences The Hebrew University of Jerusalem Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois Menahem","family":"Br\u00e9g\u00e9g\u00e8re","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry Silberman Institute of Life Sciences The Hebrew University of Jerusalem Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rami","family":"Neuman","sequence":"additional","affiliation":[{"name":"Department of Cosmetic Surgery Hadassah Hospital Ein Karem Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shlomo","family":"Magdassi","sequence":"additional","affiliation":[{"name":"Casali Center for Applied Chemistry Institute of Chemistry The Hebrew University of Jerusalem Jerusalem Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoram","family":"Milner","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry Silberman Institute of Life Sciences The Hebrew University of Jerusalem Jerusalem Israel"},{"name":"Dead\u2010Sea and Arava Science Center Ein Gedi Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/09546630500423914"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)92829-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)91335-9"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(95)00265-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1990.tb08248.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2009.10.016"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2005.12.029"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2004.12.003"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2006.02.003"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:PHAM.0000045239.22049.70"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10717540600642431"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5173(99)00361-0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2004.02.028"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2006.07.022"},{"key":"e_1_2_7_16_1","first-page":"191","volume":"64","author":"Liu H Z","year":"2010","journal-title":"PDA J Pharm Sci Technol"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016015803611"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2011.01251.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2011.01317.x"},{"key":"e_1_2_7_20_1","unstructured":"SorokaY Fru\u0161i\u0107\u2010ZlotkinM VerkhovskyLet\u00a0al.EGF and LPS\u2010stimulated skin organ culture displays inflammatory and psoriatic features: a model for biological evaluation of topical drugs. 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