{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:58:52Z","timestamp":1791291532413,"version":"4.3.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,1,31]],"date-time":"2013-01-31T00:00:00Z","timestamp":1359590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pemphigus vulgaris (<jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>) is an autoimmune blistering skin disease characterized by suprabasal acantholysis produced as a consequence of desmoglein (<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg) and non\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg autoantibodies binding to several targeting molecules localized on the membrane of keratinocytes. Nitric oxide (<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>) may exert a pathogenic function in several immunological processes. We have previously demonstrated that neural nitric oxide synthase (<jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content>) plays part in <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> acantholysis. Also, our group has described a relevant role for <jats:styled-content style=\"fixed-case\">HER<\/jats:styled-content> [human epidermal growth factor receptor (<jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>) related] isoforms and several kinases such as Src (Rous sarcoma), mammalian target of rapamycin (<jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content>) and focal adhesion kinase (<jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content>), as well as caspases in <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> development. Using a passive transfer mouse model of <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>, we aimed to investigate the relationship between the increase in <jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>, Src, <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content> kinase upregulation observed in <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> lesions. Our results revealed a new function for <jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content>, which contributes to <jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>\u2010mediated <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> acantholysis through the upregulation of Src, <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content>. In addition, we found that <jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content> participates actively in <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> at least in part by increasing caspase\u20109 and caspase\u20103 activities. These findings underline the important issue that in <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> acantholysis, caspase activation is a <jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content>\u2010linked process downstream of Src, <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content> kinase upregulation.<\/jats:p>","DOI":"10.1111\/exd.12088","type":"journal-article","created":{"date-parts":[[2013,1,17]],"date-time":"2013-01-17T16:24:01Z","timestamp":1358439841000},"page":"125-130","source":"Crossref","is-referenced-by-count":19,"title":["Neural nitric oxide synthase participates in pemphigus vulgaris acantholysis through upregulation of Rous sarcoma, mammalian target of rapamycin and focal adhesion kinase"],"prefix":"10.1111","volume":"22","author":[{"given":"Agust\u00edn","family":"Espa\u00f1a","sequence":"first","affiliation":[{"name":"Department of Dermatology University Clinic of Navarra School of Medicine University of Navarra Navarra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"M\u00f2dol","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Clinic of Navarra School of Medicine University of Navarra Navarra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria P.","family":"Gil","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Clinic of Navarra School of Medicine University of Navarra Navarra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J.","family":"L\u00f3pez\u2010Zabalza","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University Clinic of Navarra School of Medicine University of Navarra Navarra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114036"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M611365200"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10305"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.081570"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2005.00367.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2011.606444"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12319948"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12613469"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2004.06147.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501365200"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.00893.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200605125"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00075.2010"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2005.12.003"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.03.107"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2012.01441.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/berh.2001.0196"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3570593"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60589-1"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(03)00006-9"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1089-8603(02)00002-2"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119206"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.5.2007"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03401707"},{"key":"e_1_2_9_26_1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1046\/j.1365-2133.1997.d01-1136.x","volume":"136","author":"Rowe A","year":"1997","journal-title":"Br J Dermatol"},{"issue":"2","key":"e_1_2_9_27_1","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1042\/bj2810419","volume":"281","author":"Hogg N","year":"1992","journal-title":"Biochem J"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1998.0755"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08582.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.22731.x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(99)70183-0"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2011.01899.x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.137"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/08916930600971836"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2003.12014.x"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:APPT.0000018795.05766.1f"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1313.040"},{"key":"e_1_2_9_40_1","doi-asserted-by":"crossref","first-page":"456841","DOI":"10.1155\/2010\/456841","volume":"2010","author":"Bektas M","year":"2010","journal-title":"Dermatol Res Pract"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2008.12.017"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2010.05.081"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02500.x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02155-1"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-008-0263-0"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400582"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105769200"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M508258200"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402103"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2011.01.029"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.12088","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.12088","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T12:58:11Z","timestamp":1700657891000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.12088"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,31]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/exd.12088"],"URL":"https:\/\/doi.org\/10.1111\/exd.12088","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,31]]}}}