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In this study, we wish to establish an <jats:italic>in vivo<\/jats:italic> model that allows the comparison of permeability and activity of vitamin D3 analogues in human skin. We generate a bipartite, genetic sensor technology that combines efficient lentivirus\u2010directed gene delivery to xenotransplanted human skin with vitamin D3\u2010induced expression of a luciferase reporter gene and live imaging of animals by bioluminescence imaging. Based on the induction of a transcriptional activator consisting of the vitamin D receptor fused to the Gal4 <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010binding domain, the vitamin D3\u2010responsive sensor facilitates non\u2010invasive and rapid assessment of permeability and functional properties of vitamin D3 analogues. By topical application of a panel of vitamin D3 analogues onto \u2018sensorized\u2019 human skin, the sensor produces a drug\u2010induced readout with a magnitude and persistence that allow a direct comparative analysis of different analogues. This novel genetic tool has great potential as a non\u2010invasive <jats:italic>in vivo<\/jats:italic> screening system for further development and refinement of vitamin D3 analogues.<\/jats:p>","DOI":"10.1111\/exd.12091","type":"journal-article","created":{"date-parts":[[2013,1,19]],"date-time":"2013-01-19T18:56:38Z","timestamp":1358621798000},"page":"178-183","source":"Crossref","is-referenced-by-count":4,"title":["A lentiviral vector\u2010based genetic sensor system for comparative analysis of permeability and activity of vitamin D3 analogues in xenotransplanted human skin"],"prefix":"10.1111","volume":"22","author":[{"given":"Nicklas Heine","family":"Staunstrup","sequence":"first","affiliation":[{"name":"Department of Biomedicine Aarhus University Aarhus C Denmark"},{"name":"HuaDa JiYin (BGI) Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasmus O.","family":"Bak","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Aarhus University Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujia","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Aarhus University Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Svensson","sequence":"additional","affiliation":[{"name":"Department of Disease Pharmacology LEO Pharma Ballerup Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas K.","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Disease Pharmacology LEO Pharma Ballerup Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Rosada","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Aarhus University Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Stenderup","sequence":"additional","affiliation":[{"name":"Department of 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