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Therefore, management of diabetic ulcers remains a challenging clinical issue. Within this context, reliable animal models that recapitulate situations of impaired wound healing have become essential. In this study, we established a new<jats:italic>in vivo<\/jats:italic>humanised model of delayed wound healing in a diabetic context that reproduces the main features of the human disease. Diabetes was induced by multiple low doses of streptozotocin in bioengineered human\u2010skin\u2010engrafted immunodeficient mice. The significant delay in wound closure exhibited in diabetic wounds was mainly attributed to alterations in the granulation tissue formation and resolution, involving defects in wound bed maturation, vascularisation, inflammatory response and collagen deposition. In the new model, a cell\u2010based wound therapy consisting of the application of plasma\u2010derived fibrin dermal scaffolds containing fibroblasts consistently improved the healing response by triggering granulation tissue maturation and further providing a suitable matrix for migrating keratinocytes during wound re\u2010epithelialisation. The present preclinical wound healing model was able to shed light on the biological processes responsible for the improvement achieved, and these findings can be extended for designing new therapeutic approaches with clinical relevance.<\/jats:p>","DOI":"10.1111\/exd.12097","type":"journal-article","created":{"date-parts":[[2013,1,22]],"date-time":"2013-01-22T11:06:41Z","timestamp":1358852801000},"page":"195-201","source":"Crossref","is-referenced-by-count":36,"title":["The regenerative potential of fibroblasts in a new diabetes\u2010induced delayed humanised wound healing model"],"prefix":"10.1111","volume":"22","author":[{"given":"Luc\u00eda","family":"Mart\u00ednez\u2010Santamar\u00eda","sequence":"first","affiliation":[{"name":"Department of Bioengineering Carlos III University (UC3M) Madrid Spain"},{"name":"Regenerative Medicine Unit Epithelial Biomedicine Division CIEMAT Madrid Spain"},{"name":"Centre for Biomedical Research on Rare Diseases (CIBERER) ISCIII Valencia 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