{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:17:21Z","timestamp":1791231441734,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,10,29]],"date-time":"2013-10-29T00:00:00Z","timestamp":1383004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>cl\u20102 family member <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>cl\u20101 is essential for melanoma survival; however, the influence of oncogenic <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> signalling remains elusive. In this study, <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>cl\u20101 splice variant expression was determined in a panel of melanoma cell lines in relation to <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutational status. <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>cl\u20101<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression was increased in melanoma cells compared with primary melanocytes with significantly increased m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and protein expression observed in <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><\/jats:italic><jats:sup><jats:italic>V600E<\/jats:italic><\/jats:sup> mutant melanoma cells. Although no change in <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>cl\u20101S m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was observed, <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>cl\u20101<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content> protein expression also increased in <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutant melanoma cells. Additionally, while over\u2010expression of mutant <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><jats:sup>V600E<\/jats:sup> increased both <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>cl\u20101<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>cl\u20101<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content> expression, inhibition of hyperactive <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> signalling resulted in decreased <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>cl\u20101<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content> expression. These studies suggest that the regulation of <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>cl\u20101 expression by <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> signalling is increased by oncogenic activation of <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content>, revealing a mechanism of apoptotic resistance which may be overcome by the use of more specifically targeted <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>cl\u20101 inhibitors.<\/jats:p>","DOI":"10.1111\/exd.12254","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T01:42:19Z","timestamp":1381542139000},"page":"767-769","source":"Crossref","is-referenced-by-count":39,"title":["Oncogenic <scp>BRAF<\/scp> signalling increases <scp>M<\/scp>cl\u20101 expression in cutaneous metastatic melanoma"],"prefix":"10.1111","volume":"22","author":[{"given":"Christopher S.","family":"McKee","sequence":"first","affiliation":[{"name":"Dermatological Sciences Institute of Cellular Medicine Newcastle University Newcastle Upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Hill","sequence":"additional","affiliation":[{"name":"Dermatological Sciences Institute of Cellular Medicine Newcastle University Newcastle Upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher P. F.","family":"Redfern","sequence":"additional","affiliation":[{"name":"Northern Institute for Cancer Research Newcastle University Newcastle Upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane L.","family":"Armstrong","sequence":"additional","affiliation":[{"name":"Faculty of Applied Sciences University of Sunderland Sunderland UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penny E.","family":"Lovat","sequence":"additional","affiliation":[{"name":"Dermatological Sciences Institute of Cellular Medicine Newcastle University Newcastle Upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,10,29]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)17951-3"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1002011"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(12)70539-9"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.06.069"},{"key":"e_1_2_5_6_1","first-page":"1865","volume":"4","author":"Tang L","year":"1998","journal-title":"Clin Cancer Res"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M909826199"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M909572199"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.83"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm301448p"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051497"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2011.01385.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207692"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M708426200"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20041596"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.12254","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.12254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T07:10:11Z","timestamp":1696317011000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.12254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,29]]},"references-count":14,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1111\/exd.12254"],"URL":"https:\/\/doi.org\/10.1111\/exd.12254","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,29]]}}}