{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T15:34:03Z","timestamp":1757777643619},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,10,29]],"date-time":"2013-10-29T00:00:00Z","timestamp":1383004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia<\/jats:italic> yeasts are responsible for the widely distributed skin disease Pityriasis versicolor (<jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>), which is characterized by a hyper\u2010 or hypopigmentation of affected skin areas. For <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alassezia furfur<\/jats:italic>, it has been shown that pigment production relies on tryptophan metabolism. A tryptophan aminotransferase was found to catalyse the initial catalytic step in pigment formation in the model organism <jats:italic><jats:styled-content style=\"fixed-case\">U<\/jats:styled-content>stilago maydis<\/jats:italic>. Here, we describe the sequence determination, recombinant production and biochemical characterization of tryptophan aminotransferase <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>f<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>am1 from <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>.\u00a0furfur<\/jats:italic>. The enzyme catalyses the transamination from <jats:sc>l<\/jats:sc>\u2010tryptophan to keto acids such as \u03b1\u2010ketoglutarate with <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> values for both substrates in the low millimolar range. Furthermore, <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>f<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>am1 presents a temperature optimum at 40\u00b0C and a p<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> optimum at 8.0. <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>f<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>am1 activity is highly dependent on pyridoxal phosphate (<jats:styled-content style=\"fixed-case\">PLP<\/jats:styled-content>), whereas compounds interfering with <jats:styled-content style=\"fixed-case\">PLP<\/jats:styled-content>, such as cycloserine (<jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>) and aminooxyacetate, inhibit the <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>f<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>am1 reaction. <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content> is known to reverse hyperpigmentation in <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>. Thus, the results of the present study give a deeper insight into the role of <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>f<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>am1 in <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> pathogenesis and as potential target for the development of novel <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> therapeutics.<\/jats:p>","DOI":"10.1111\/exd.12260","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T02:13:53Z","timestamp":1381544033000},"page":"736-741","source":"Crossref","is-referenced-by-count":14,"title":["Characterization of tryptophan aminotransferase 1 of <i><scp>M<\/scp>alassezia furfur<\/i>, the key enzyme in the production of indolic compounds by <i>M.\u00a0furfur<\/i>"],"prefix":"10.1111","volume":"22","author":[{"given":"Janina","family":"Preuss","sequence":"first","affiliation":[{"name":"Center for Dermatology, 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