{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:23:10Z","timestamp":1790828590227,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,12,30]],"date-time":"2013-12-30T00:00:00Z","timestamp":1388361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The density of peripheral nerve fibres is increased in atopic dermatitis. Moreover, reduction in the fibres in a mouse model of atopic dermatitis reduces scratching behaviour. Thus, regulation of nerve fibre extension could be an effective strategy to reduce itching in pruritus dermatosis. In this study, we established a new coculture system of keratinocytes and dorsal\u2010root\u2010ganglion\u2010derived cells using an apparatus, <jats:styled-content style=\"fixed-case\">AXIS<\/jats:styled-content><jats:sup>\u2122<\/jats:sup>, which consists of two different channels connected via a set of microgrooves, through which signalling molecules and axons, but not living cells, can pass. When we seeded keratinocytes in one chamber, extension of nerve fibres was observed from dorsal root ganglion cells seeded in the other chamber. Addition of anti\u2010<jats:styled-content style=\"fixed-case\">BDNF<\/jats:styled-content> antibody in the keratinocyte\u2010seeded chamber significantly reduced the extension. Application of Semaphorin 3A also reduced the extension by approximately 50%. We suggest that this coculture system may be useful for screening of anti\u2010itching drugs.<\/jats:p>","DOI":"10.1111\/exd.12288","type":"journal-article","created":{"date-parts":[[2013,11,25]],"date-time":"2013-11-25T10:32:40Z","timestamp":1385375560000},"page":"58-60","source":"Crossref","is-referenced-by-count":21,"title":["Coculture system of keratinocytes and dorsal\u2010root\u2010ganglion\u2010derived cells for screening neurotrophic factors involved in guidance of neuronal axon growth in the skin"],"prefix":"10.1111","volume":"23","author":[{"given":"Jun\u2010ichi","family":"Kumamoto","sequence":"first","affiliation":[{"name":"CREST Japan Science and Technology Agency Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Nakatani","sequence":"additional","affiliation":[{"name":"CREST Japan Science and Technology Agency Tokyo Japan"},{"name":"Japan Society for the Promotion of Science Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moe","family":"Tsutsumi","sequence":"additional","affiliation":[{"name":"CREST Japan Science and Technology Agency Tokyo Japan"},{"name":"Shiseido Research Center Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makiko","family":"Goto","sequence":"additional","affiliation":[{"name":"CREST Japan Science and Technology Agency Tokyo Japan"},{"name":"Shiseido Research Center Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumiko","family":"Denda","sequence":"additional","affiliation":[{"name":"CREST Japan Science and Technology Agency Tokyo Japan"},{"name":"Shiseido Research Center Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Takei","sequence":"additional","affiliation":[{"name":"CREST Japan Science and Technology Agency Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuhiro","family":"Denda","sequence":"additional","affiliation":[{"name":"CREST Japan Science and Technology Agency Tokyo Japan"},{"name":"Shiseido Research Center Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2006.00529.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2009.03.007"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2008.08.011"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12107"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2011.01276.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12019"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.316"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2007.05649.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.44"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.10.4516"},{"key":"e_1_2_8_12_1","first-page":"557","volume":"79","author":"Botchkarev V A","year":"1999","journal-title":"Lab Invest"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0563-07.2007"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(93)90223-L"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.150"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.12.015"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.12288","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.12288","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T16:41:37Z","timestamp":1695573697000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.12288"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,30]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/exd.12288"],"URL":"https:\/\/doi.org\/10.1111\/exd.12288","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,30]]}}}