{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:00:12Z","timestamp":1791212412935,"version":"4.1.0"},"reference-count":5,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,12,30]],"date-time":"2013-12-30T00:00:00Z","timestamp":1388361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have identified a spliced <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> transcript of CD20 (named D393\u2010CD20) which was associated with resistance to RTX in primary B cell from patients with lymphoma and leukaemia. In the present work, we wished to investigate whether D393\u2010CD20 variant was expressed by B cells from patients with pemphigus. Ten patients with bullous pemphigoid and twenty\u2010five patients with pemphigus were included. All patients were responder to conventional immunosuppressive agents or rituximab (<jats:italic>n<\/jats:italic>\u00a0=\u00a011). Efficacy of B\u2010cell activation by pokeweed mitogen was assessed by CD86 expression using a FACS Canto II flow cytometer. <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> CD20 expression study was then performed using RT\u2010PCR assay allowing first to discriminate wild\u2010type (wt)\u2010CD20 and D393\u2010CD20 transcript. Although wt\u2010CD20 expression was always detected, we were unable to detect D393\u2010CD20, even after B\u2010cell activation or RTX treatment. Our results suggest that D393\u2010CD20 transcript may be a molecular marker of B\u2010cell malignancies rather than autoimmune disease like pemphigus. Further study of RTX non\u2010responders or non\u2010escaping PV patients is thus still required to appreciate whether D393\u2010CD20 expression may be detected under the pressure of RTX therapy.<\/jats:p>","DOI":"10.1111\/exd.12299","type":"journal-article","created":{"date-parts":[[2013,12,7]],"date-time":"2013-12-07T15:10:49Z","timestamp":1386429049000},"page":"66-67","source":"Crossref","is-referenced-by-count":17,"title":["Lack of expression of an alternative <scp>CD<\/scp>20 transcript variant in circulating B cells from patients with pemphigus"],"prefix":"10.1111","volume":"23","author":[{"given":"Cl\u00e9mentine","family":"Gamonet","sequence":"first","affiliation":[{"name":"Inserm UMR 1098 Etablissement Fran\u00e7ais du Sang Bourgogne\u2010Franche Comt\u00e9 Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Ferrand","sequence":"additional","affiliation":[{"name":"Inserm UMR 1098 Etablissement Fran\u00e7ais du Sang Bourgogne\u2010Franche Comt\u00e9 Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natacha","family":"Colliou","sequence":"additional","affiliation":[{"name":"Clinique Dermatologique H\u00f4pital Charles Nicolle Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Musette","sequence":"additional","affiliation":[{"name":"Clinique Dermatologique H\u00f4pital Charles Nicolle Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Joly","sequence":"additional","affiliation":[{"name":"Clinique Dermatologique H\u00f4pital Charles Nicolle Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Girardin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Franche Comt\u00e9 EA3181 SFR 4234 et Service de Dermatologie CHU de Besan\u00e7on Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Humbert","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Franche Comt\u00e9 Inserm UMR1098 SFR 4234 et Service de Dermatologie CHU de Besan\u00e7on Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Aubin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Franche Comt\u00e9 EA3181 SFR 4234 et Service de Dermatologie CHU de Besan\u00e7on Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12088"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa067752"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04744.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-229112"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/10430340050015202"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.12299","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.12299","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T16:41:49Z","timestamp":1695573709000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.12299"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,30]]},"references-count":5,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/exd.12299"],"URL":"https:\/\/doi.org\/10.1111\/exd.12299","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,30]]}}}