{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T08:58:59Z","timestamp":1790499539013,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,3,28]],"date-time":"2014-03-28T00:00:00Z","timestamp":1395964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pemphigus foliaceus (<jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content>) and pemphigus vulgaris (<jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>) are life\u2010threatening autoimmune blistering skin diseases. They are characterized by circulating autoantibodies which bind to the ectodomains of desmoglein (Dsg) 1 and <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg3. These antibodies induce acantholysis in skin and mucous membranes. In severe cases of pemphigus, immunoadsorption is applied to remove total IgG from patient plasma using protein A or other ligands. To develop a specific adsorber for anti\u2010Dsg antibodies, epitope mapping studies of <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg1 and <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg3 ectodomains were conducted. Dsg variants were expressed on the surface of <jats:styled-content style=\"fixed-case\">HEK<\/jats:styled-content>\u2010293 cells and analysed for reactivity with pemphigus and control sera by indirect immunofluorescence technique. For Dsg1, a construct consisting of domain 1 directly fused to domain 5, seemed to be suitable for specific immunoadsorption of anti\u2010Dsg1 antibodies. The recognized epitopes were mainly conformation\u2010dependent. However, adsorption of pemphigus foliaceus IgG using this protein coupled to a Sepharose matrix did not completely remove pathogenicity from the sera, as proven by a keratinocyte dissociation assay. In contrast, full\u2010length Dsg1 and Dsg3 ectodomains were able to specifically adsorb anti\u2010Dsg antibodies and to efficiently eliminate pathogenicity. Therefore, the complete and correctly folded ectodomains of both desmogleins are required for therapeutic immunoadsorption.<\/jats:p>","DOI":"10.1111\/exd.12355","type":"journal-article","created":{"date-parts":[[2014,2,18]],"date-time":"2014-02-18T02:51:00Z","timestamp":1392691860000},"page":"253-259","source":"Crossref","is-referenced-by-count":29,"title":["Specific immunoadsorption of pathogenic autoantibodies in pemphigus requires the entire ectodomains of desmogleins"],"prefix":"10.1111","volume":"23","author":[{"given":"Jana","family":"Langenhan","sequence":"first","affiliation":[{"name":"Institute of Experimental Immunology Euroimmun AG L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Dworschak","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck L\u00fcbeck 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