{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T05:11:29Z","timestamp":1695618689999},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,5,28]],"date-time":"2014-05-28T00:00:00Z","timestamp":1401235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated protein expression and <jats:italic>in situ<\/jats:italic> activity of transglutaminases (<jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>s) in normal skin and various epidermal neoplasms. In normal skin, <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>1 protein expression and <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> activity were found at keratinocyte cell membranes in upper epidermis and granular layer, respectively. In seborrhoeic keratosis, <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>1 protein was expressed evenly throughout tumors, while <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> activity increased in gradient fashion from lower tumor area to cornified layer. In squamous cell carcinoma, <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>1 protein was expressed at inner side of cell membranes, whereas <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> activity was found in cytoplasm predominantly at horn pearls. In basal cell carcinoma, weak <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> activity was found in cytoplasm of all tumor cells without the presence of <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>1 protein. Immunoblotting and <jats:italic>in situ<\/jats:italic> activity assays using specific substrate peptides confirmed that <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>2, but not <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>1, contributed to the <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content> activity. These results suggested that different expression and activation of <jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>s may contribute to characteristics of the skin tumors.<\/jats:p>","DOI":"10.1111\/exd.12418","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T08:34:43Z","timestamp":1398069283000},"page":"433-435","source":"Crossref","is-referenced-by-count":5,"title":["Distinct protein expression and activity of transglutaminases found in different epidermal tumors"],"prefix":"10.1111","volume":"23","author":[{"given":"Tadashi","family":"Karashima","sequence":"first","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minao","family":"Furumura","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norito","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bungo","family":"Ohyama","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Saruta","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohei","family":"Natsuaki","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takekuni","family":"Nakama","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chika","family":"Ohata","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Tsuruta","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyotaka","family":"Hitomi","sequence":"additional","affiliation":[{"name":"Department of Applied Molecular Biosciences Graduate School of Pharmaceutical Sciences Nagoya University Chikusa Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,28]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"313","volume":"15","author":"Hitomi K","year":"2005","journal-title":"Dermatol"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2013.06.010"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.3.1044"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12373021"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.44.26382"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010157200"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90217-X"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2162"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/4.11.1413"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1989.tb01569.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394305-7.00001-X"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.464693"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-014-1688-0"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0832466100"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)98617-5"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00044.2008"},{"key":"e_1_2_5_18_1","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1002\/ijc.2910600319","volume":"60","author":"Groningen J J","year":"1995","journal-title":"Int J Cancer"},{"key":"e_1_2_5_19_1","first-page":"637","volume":"75","author":"Hettasch J M","year":"1996","journal-title":"Lab Invest"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-012-2351-7"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-012-1229-7"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-4-33"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06692.x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2010.957225"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12612769"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00376822"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00116.x"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12075"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.12418","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.12418","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T09:15:02Z","timestamp":1695546902000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.12418"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,28]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1111\/exd.12418"],"URL":"https:\/\/doi.org\/10.1111\/exd.12418","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,28]]}}}