{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:46:44Z","timestamp":1790880404876,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T00:00:00Z","timestamp":1405468800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Detection of a dominant T\u2010cell clone by T\u2010cell receptor (<jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>) gene rearrangement analysis is often essential for the diagnosis of cutaneous T\u2010cell lymphomas (<jats:styled-content style=\"fixed-case\">CTCL<\/jats:styled-content>). The occurrence of T\u2010cell clones in addition to the diagnostic T\u2010cell clone during the course of <jats:styled-content style=\"fixed-case\">CTCL<\/jats:styled-content> has been reported, but the data of these studies have been contradictory. We retrospectively evaluated the data of 114 lesional skin biopsies from 26 patients with Mycosis fungoides and two patients with primary cutaneous anaplastic large cell lymphoma, which were analysed with the standardized Biomed\u20102 <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for the <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content><jats:italic>\u03b3<\/jats:italic> and <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content><jats:italic>\u03b2<\/jats:italic> locus. A dominant T\u2010cell clone was repetitively detected in 93% (26\/28) of patients. Additional T\u2010cell clones appeared temporarily in 39% (11\/28) of patients. Correlation with the clinical data did not show an association of the presence of additional T\u2010cell clones with age, number of treatments, progression of disease or survival. Our findings demonstrate that a persistent T\u2010cell clone, most likely the disease causing tumor clone, is detectable in almost all <jats:styled-content style=\"fixed-case\">CTCL<\/jats:styled-content> patients. In addition, transiently appearing T\u2010cell clones frequently occur during the course of disease. The biological relevance of these additional clones has still to be determined. However, it is important to take the possibility of additional T\u2010cell clones into account for diagnostic analyses.<\/jats:p>","DOI":"10.1111\/exd.12453","type":"journal-article","created":{"date-parts":[[2014,5,24]],"date-time":"2014-05-24T09:36:46Z","timestamp":1400924206000},"page":"504-508","source":"Crossref","is-referenced-by-count":11,"title":["T\u2010cell receptor gene rearrangement analysis of sequential biopsies in cutaneous T\u2010cell lymphomas with the Biomed\u20102 <scp>PCR<\/scp> reveals transient T\u2010cell clones in addition to the tumor clone"],"prefix":"10.1111","volume":"23","author":[{"given":"Daniel","family":"Humme","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergy Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ansgar","family":"Lukowsky","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Charit\u00e9 \u2013 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