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Although we previously showed that preformed autoreactive plasma cells do not seem to be directly affected by anti\u2010Hsp90 treatment, immunomodulatory effects of Hsp90 inhibition on (auto\u2010)antibody responses are not yet fully understood. In this study, the Hsp90 blocker 17\u2010<jats:styled-content style=\"fixed-case\">DMAG<\/jats:styled-content> inhibited proliferation of activated total B cells and their IgG secretion in cultures of human peripheral B cells from healthy subjects, but IgG production was no longer affected when these activated B cells were allowed to differentiate prior to a deferred application of the inhibitor. 17\u2010<jats:styled-content style=\"fixed-case\">DMAG<\/jats:styled-content> treatment was associated with induction of nuclear and cytoplasmic heat shock factor 1 and Hsp70 in stimulated human B cells, respectively. Type <jats:styled-content style=\"fixed-case\">VII<\/jats:styled-content> collagen (epidermolysis bullosa acquisita)\u2010immunized mice early treated with 17\u2010<jats:styled-content style=\"fixed-case\">DMAG<\/jats:styled-content> had reduced total B cells in spleens, a relative increase in splenic regulatory B cell fractions, higher serum <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 concentrations, and lower levels of circulating autoantibodies (paralleled by less pronounced disease induction) compared with vehicle\u2010treated immunized mice. Autoantibody production was blunted in isolated and autoantigen\u2010restimulated lymph node cells from immunized mice by either 17\u2010<jats:styled-content style=\"fixed-case\">DMAG<\/jats:styled-content> or purified autologous splenic regulatory B cells. Thus, in addition to the previously described T cell inhibitory effects of Hsp90 blockade, this treatment potently modulates humoral immune responses at the B cell level, further supporting the introduction of Hsp90 inhibitors into the clinical setting for treatment of autoantibody\u2010mediated disorders.<\/jats:p>","DOI":"10.1111\/exd.12476","type":"journal-article","created":{"date-parts":[[2014,6,24]],"date-time":"2014-06-24T16:29:04Z","timestamp":1403627344000},"page":"585-590","source":"Crossref","is-referenced-by-count":38,"title":["Immunomodulatory effects of heat shock protein 90 inhibition on humoral immune responses"],"prefix":"10.1111","volume":"23","author":[{"given":"Stefan","family":"Tukaj","sequence":"first","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Tiburzy","sequence":"additional","affiliation":[{"name":"Institute for Systemic 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