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The equine hoof represents an extreme evolutionary adaptation of an epidermal structure homologous to the human or murine nail units. Immunohistochemistry is frequently utilized in the study of the pathophysiology of laminitis. The complex, multilayered, extensively interdigitated epidermal\u2013dermal lamellar interface renders precise interpretation of immunofluorescence localization difficult, especially when effective technique and reagents render non\u2010reactive tissues completely dark. Fluorescent\u2010conjugated wheat germ agglutinin (<jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content>) selectively labels dermal extracellular matrix fibres and epidermal cell membranes in tissue sections of horse hoof lamellae, is compatible with indirect immunofluorescence and augments interpretation of indirect immunofluorescence antigen localization. The current report details the use of <jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content> as a rapid, simple, economical counterstain for immunofluorescence studies of the equine hoof and may have application to other complex epidermal tissue structures.<\/jats:p>","DOI":"10.1111\/exd.12495","type":"journal-article","created":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T15:37:41Z","timestamp":1405525061000},"page":"677-678","source":"Crossref","is-referenced-by-count":6,"title":["Wheat germ agglutinin as a counterstain for immunofluorescence studies of equine hoof lamellae"],"prefix":"10.1111","volume":"23","author":[{"given":"Robert K.","family":"Clark","sequence":"first","affiliation":[{"name":"Department of Clinical Studies New Bolton Center University of Pennsylvania School of Veterinary Medicine Kennett Square PA USA"},{"name":"STEM and Health Division Cumberland County College Vineland NJ USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah L.","family":"Galantino\u2010Homer","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies New Bolton Center University of Pennsylvania School of Veterinary Medicine Kennett Square PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ctep.2004.07.001"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2010.01.005"},{"key":"e_1_2_8_4_1","first-page":"102","volume-title":"Equine Podiatry","author":"Floyd AE","year":"2007"},{"key":"e_1_2_8_5_1","unstructured":"NAHMS.Lameness and Laminitis in U.S. Horses. 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