{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T13:56:02Z","timestamp":1778853362706,"version":"3.51.4"},"reference-count":9,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,11,25]],"date-time":"2014-11-25T00:00:00Z","timestamp":1416873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Deregulated signalling through phosphatidylinositol 3\u2010kinase (<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>3K) pathway plays a critical role in tumour initiation and progression. We have already shown that <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content> is activated in skin lesions in Mycosis Fungoides (<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>) and we herein further investigate the frequency and clinical significance of <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>3K at the protein and at the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> level as well as the presence of <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>1 mutations in skin lesions from 50 patients with <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> clinical stages I\u2010<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content> in relation to clinicopathological features. Increased p\u2010<jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content> expression correlated with poor prognosis in plaques (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0198), whereas p\u2010<jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content> was an independent predictor of poor survival in the entire cohort (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.017, <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>\u00a0=\u00a01.012). <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> cytoplasmic expression was found low or absent in all 77.3% of cases and inversely correlated with advanced clinical stages (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0744). Molecular analysis showed no <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>1 mutation, no <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>3<jats:styled-content style=\"fixed-case\">KCA<\/jats:styled-content> copy number gain, only 1 case with <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>3<jats:styled-content style=\"fixed-case\">KCA<\/jats:styled-content> mutation in exon 9 and 3 cases with <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> mutations (7%) in exons 7, 8 and 5. The latter correlated with disease (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0253) and progression (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001) free survival in tumour stage. Although activation of <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>3K\/<jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content> signalling pathway due to <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> alterations is rarely attributed to abnormalities in <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PI<\/jats:styled-content>3K, and <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>1 genes, <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> mutations exert a negative effect on patients\u2019 prognosis with tumours.<\/jats:p>","DOI":"10.1111\/exd.12547","type":"journal-article","created":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T06:38:19Z","timestamp":1412059099000},"page":"931-933","source":"Crossref","is-referenced-by-count":16,"title":["<i>In situ<\/i> assessment of <scp>PI<\/scp>3K and PTEN alterations in mycosis fungoides: correlation with clinicopathological features"],"prefix":"10.1111","volume":"23","author":[{"given":"Evangelia","family":"Papadavid","sequence":"first","affiliation":[{"name":"1st Department of Dermatology Cutaneous lymphoma Clinic A Syngros Hospital University of Athens, Medical School Athens Greece"},{"name":"2d Department of Dermatology Cutaneous Lymphoma Clinic ATTIKON University General Hospital University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Penelope","family":"Korkolopoulou","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgia","family":"Levidou","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelica A.","family":"Saetta","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodora","family":"Papadaki","sequence":"additional","affiliation":[{"name":"Heamatolopathology Department Evangelismos General Hospital Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Siakantaris","sequence":"additional","affiliation":[{"name":"1st Department of Internal Medicine Laikon Hospital University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassiliki","family":"Nikolaou","sequence":"additional","affiliation":[{"name":"1st Department of Dermatology Cutaneous lymphoma Clinic A Syngros Hospital University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afroditi","family":"Oikonomidi","sequence":"additional","affiliation":[{"name":"1st Department of Dermatology Cutaneous lymphoma Clinic A Syngros Hospital University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilenia","family":"Chatziandreou","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonidas","family":"Marinos","sequence":"additional","affiliation":[{"name":"Heamatolopathology Department Evangelismos General Hospital Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aggeliki","family":"Kolialexi","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics Agia Sofia Hospital for Children University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandros","family":"Stratigos","sequence":"additional","affiliation":[{"name":"1st Department of Dermatology Cutaneous lymphoma Clinic A Syngros Hospital University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrios","family":"Rigopoulos","sequence":"additional","affiliation":[{"name":"2d Department of Dermatology Cutaneous Lymphoma Clinic ATTIKON University General Hospital University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanta","family":"Psyrri","sequence":"additional","affiliation":[{"name":"Division of Oncology 2nd Propaideutic Department of Internal Medicine ATTIKON University General Hospital University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eftratios","family":"Patsouris","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Antoniou","sequence":"additional","affiliation":[{"name":"1st Department of Dermatology Cutaneous lymphoma Clinic A Syngros Hospital University of Athens, Medical School Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,11,25]]},"reference":[{"key":"e_1_2_2_2_1","first-page":"667","volume":"996","author":"Osaki M","year":"2005","journal-title":"Apoptosis"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc839"},{"key":"e_1_2_2_4_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1146\/annurev.pathol.4.110807.092311","volume":"4","author":"Chaloud N","year":"2009","journal-title":"Annu Rev Pathol"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-510578"},{"key":"e_1_2_2_6_1","doi-asserted-by":"crossref","first-page":"2937","DOI":"10.1182\/blood.V95.9.2937.009k15_2937_2942","volume":"95","author":"Scarisbrick J J","year":"2000","journal-title":"Blood"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2013.04.027"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04523.x"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2006.02.024"},{"key":"e_1_2_2_10_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3324\/haematol.2012.068510","volume":"98","author":"Martin\u2010Sanchez E","year":"2012","journal-title":"Haematologica"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.12547","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.12547","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T06:26:21Z","timestamp":1695536781000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.12547"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,25]]},"references-count":9,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1111\/exd.12547"],"URL":"https:\/\/doi.org\/10.1111\/exd.12547","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,25]]}}}