{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T06:02:31Z","timestamp":1783576951774,"version":"3.55.0"},"reference-count":9,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,3,27]],"date-time":"2015-03-27T00:00:00Z","timestamp":1427414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81271758"],"award-info":[{"award-number":["81271758"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472887"],"award-info":[{"award-number":["81472887"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NSF of Zhejiang","award":["LY13H110001"],"award-info":[{"award-number":["LY13H110001"]}]},{"name":"NSF of Zhejiang","award":["Y2111310"],"award-info":[{"award-number":["Y2111310"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Considerable evidence implicates that viral infection might be a participant factor in the pathogenesis of vitiligo. However, it is still unclear how viral infection leads to the melanocyte destruction. To elucidate the effects of viral ds<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> on the viability and cytokine synthesis of normal human melanocytes and to explore the underlying mechanisms, primary cultured normal human melanocytes were transfected with poly(<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">dT<\/jats:styled-content>). The results demonstrated that poly(<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">dT<\/jats:styled-content>) triggered apoptosis instead of pyroptosis in melanocytes. Knocking down <jats:styled-content style=\"fixed-case\">AIM<\/jats:styled-content>2 or <jats:styled-content style=\"fixed-case\">RIG<\/jats:styled-content>\u2010I by <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> interference partially reduced the poly(<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">dT<\/jats:styled-content>)\u2010induced <jats:styled-content style=\"fixed-case\">LDH<\/jats:styled-content> release, suggesting the involvement of both nucleic acid sensors in the process of melanocyte death. Poly(<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">dT<\/jats:styled-content>) induced the expression of pro\u2010inflammatory cytokine genes including <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b2, <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 as well, whereas the pro\u2010inflammatory cytokine production was suppressed by <jats:styled-content style=\"fixed-case\">RIG<\/jats:styled-content>\u2010I si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, but not by <jats:styled-content style=\"fixed-case\">AIM<\/jats:styled-content>2 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. Poly(<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">dT<\/jats:styled-content>) treatment increased the phosphorylation of p38 and <jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u03baB. Accordingly, <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u03baB inhibitor Bay 11\u20107082 and <jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content> inhibitor <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>600125 blocked the induction of the cytokine genes except <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b2. The production of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>6 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>8 was also suppressed by p38 inhibitor <jats:styled-content style=\"fixed-case\">SB<\/jats:styled-content>203580. On the contrary, the Poly(<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">dT<\/jats:styled-content>)\u2010induced melanocyte death was only decreased by <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>600125. This study provides the possible mechanism of melanocyte destruction and immuno\u2010stimulation in vitiligo by innate immune response following viral infection.<\/jats:p>","DOI":"10.1111\/exd.12621","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T06:23:33Z","timestamp":1418797413000},"page":"298-300","source":"Crossref","is-referenced-by-count":25,"title":["Cytosolic ds<scp>DNA<\/scp> triggers apoptosis and pro\u2010inflammatory cytokine production in normal human melanocytes"],"prefix":"10.1111","volume":"24","author":[{"given":"Suiquan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Dermatology Third People's Hospital of Hangzhou Hangzhou Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongyin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Third People's Hospital of Hangzhou Hangzhou Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weixuan","family":"Ning","sequence":"additional","affiliation":[{"name":"Department of Dermatology Third People's Hospital of Hangzhou Hangzhou Zhejiang Province China"},{"name":"Guangxing Hospital Zhejiang Chinese Medical University Hangzhou Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aie","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Third People's Hospital of Hangzhou Hangzhou Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,27]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12103"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12183"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2000.130411.x"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2001.140107.x"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01044.x"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3030"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.181"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1331.2009.02563.x"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.11.7497"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.12621","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.12621","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T13:43:17Z","timestamp":1695562997000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.12621"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,27]]},"references-count":9,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1111\/exd.12621"],"URL":"https:\/\/doi.org\/10.1111\/exd.12621","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,27]]}}}