{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T20:33:50Z","timestamp":1774557230374,"version":"3.50.1"},"reference-count":9,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,3,27]],"date-time":"2015-03-27T00:00:00Z","timestamp":1427414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2015,4]]},"abstract":"Abstract<\/jats:title>Experiments were conducted to develop a model to study the effect of oral and topical administration of theNK<\/jats:styled-content>1 receptor antagonist aprepitant, on scratching behaviour in gerbils. The gerbil was selected due to its relevance for humanNK<\/jats:styled-content>1 receptor pharmacology. Intradermal injection of a specificNK<\/jats:styled-content>1 receptor agonistGR<\/jats:styled-content>73632 (100\u00a0nmol\/100\u00a0\u00b5l) at the rostral back of gerbils produced scratching of the injection site. This could be attenuated by intradermal co\u2010administration of a selectiveNK<\/jats:styled-content>1 receptor antagonist aprepitant (30\u2013100\u2013300\u00a0nmol), demonstrating the role of dermalNK<\/jats:styled-content>1 receptor in elicitation of scratching behaviour. Likewise, scratching was attenuated by oral (0.3\u20133\u201330\u00a0mg\/kg) or topical application (0.01\u20130.1\u20131% w\/v) of aprepitant and pharmacokinetic analysis of aprepitant levels in brain, blood and skin supported that efficacy of topically applied aprepitant was due to dermal rather than central target engagement. In conclusion, we showed thatNK<\/jats:styled-content>1 agonist\u2010induced scratching in the gerbil can be reversed by systemic and topical administration of aprepitant. This test system may provide a useful model for thein vivo<\/jats:italic>assessment of putative antipruritic agents.<\/jats:p>","DOI":"10.1111\/exd.12656","type":"journal-article","created":{"date-parts":[[2015,2,4]],"date-time":"2015-02-04T08:24:09Z","timestamp":1423038249000},"page":"312-314","source":"Crossref","is-referenced-by-count":8,"title":["TheNK<\/scp>1 receptor antagonist aprepitant attenuatesNK<\/scp>1 agonist\u2010induced scratching behaviour in the gerbil after intra\u2010dermal, topical or oral administration"],"prefix":"10.1111","volume":"24","author":[{"given":"Vivian J. A.","family":"Costantini","sequence":"first","affiliation":[{"name":"Center of Drug Design & Discovery Aptuit s.r.l. Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Corsi","sequence":"additional","affiliation":[{"name":"Center of Drug Design & Discovery Aptuit s.r.l. 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