{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:24:10Z","timestamp":1791271450171,"version":"4.1.0"},"reference-count":9,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,5,4]],"date-time":"2015-05-04T00:00:00Z","timestamp":1430697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Helios is a member of the Ikaros transcription factor family and has been reported to be a marker of thymus\u2010derived regulatory T cells (Treg). Helios is an intracellular protein, however, and hence cannot be used as a marker to separate living Tregs. To solve this problem, we generated Helios reporter mice in which Helios+ cells selectively express Venus, a variant of green fluorescent protein. Most of the Tregs in the thymus expressed Helios, whereas its expression was varied in peripheral lymphoid organs. The Helios+ Treg\u2010population was superior in ability to suppress both antigen\u2010specific and <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>\u2010stimulated T cell responses. We also showed that Helios+ Tregs inhibited the cytokine production by T cells more efficiently than Helios\u2010 Tregs. We conclude that Helios reporter mouse strain is a useful tool to study function of Helios and that Helios+ Tregs represent the highly suppressive population.<\/jats:p>","DOI":"10.1111\/exd.12711","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T18:15:24Z","timestamp":1427912124000},"page":"554-556","source":"Crossref","is-referenced-by-count":30,"title":["Generation of Helios reporter mice and an evaluation of the suppressive capacity of Helios<sup>+<\/sup> regulatory T cells <i>in\u00a0vitro<\/i>"],"prefix":"10.1111","volume":"24","author":[{"given":"Kazunari","family":"Sugita","sequence":"first","affiliation":[{"name":"Department of Dermatology Kyoto University Graduate School of Medicine Kyoto Japan"},{"name":"Department of Dermatology University of Occupational and Environmental Health Kitakyushu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Hanakawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kyoto University Graduate School of Medicine Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Honda","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kyoto University Graduate School of Medicine Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gen","family":"Kondoh","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Experiments for Regeneration Institute for Frontier Medical Sciences Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiki","family":"Miyachi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kyoto University Graduate School of Medicine Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Kabashima","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kyoto University Graduate School of Medicine Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Nomura","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kyoto University Graduate School of Medicine Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,4]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-011-0846-8"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.284"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI40926"},{"key":"e_1_2_2_5_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jdermsci.2011.06.004","volume":"63","author":"Honda T","year":"2011","journal-title":"J Dermatol Sci"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2006.00427.x"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2010.02.001"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0904028"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0913256107"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12044"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.12711","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.12711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T16:02:56Z","timestamp":1695571376000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.12711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,4]]},"references-count":9,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1111\/exd.12711"],"URL":"https:\/\/doi.org\/10.1111\/exd.12711","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,4]]}}}