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Reduced decorin synthesis is associated with dermal scarring, and increased decorin expression appears to reduce scar tissue formation. To investigate the therapeutic potential of decorin for keloids, human dermal fibroblasts (HDFs) and keloid\u2010derived fibroblasts (KFs) were transduced with decorin\u2010expressing adenovirus (dE1\u2010RGD\/GFP\/DCN), and we examined the therapeutic potential of decorin\u2010expressing Ad for treating pathologic skin fibrosis. Decorin expression was examined by immunofluorescence assay on keloid tissues. HDFs and KFs were transduced with dE1\u2010RGD\/GFP\/DCN or control virus, and protein levels of decorin, epidermal growth factor receptor (EGFR) and secreted TGF\u2010\u03b21 were assessed by Western blotting and ELISA. And type I and III collagen, and matrix metalloproteinase\u20101 (MMP\u20101) and matrix metalloproteinase\u20103 (MMP\u20103) mRNA levels were measured by real\u2010time RT\u2010PCR. Additionally, we immunohistochemically investigated the expression levels of the major extracellular matrix (ECM) proteins in keloid spheroids transduced with dE1\u2010RGD\/GFP\/DCN. Lower decorin expression was observed in the keloid region compared to adjacent normal tissues. After treatment with dE1\u2010RGD\/GFP\/DCN, secreted TGF\u2010\u03b21 and EGFR protein expressions were decreased in TGF\u2010\u03b21\u2010treated HDFs and KFs. Also, type I and III collagen mRNA levels were decreased, and the expression of MMP\u20101 and MMP\u20103 mRNA was strongly upregulated. In addition, the expression of type I and III collagen, fibronectin and elastin was significantly reduced in dE1\u2010RGD\/GFP\/DCN\u2010transduced keloid spheroids. These results support the utility of decorin\u2010expressing adenovirus to reduce collagen synthesis in KFs and keloid spheroid, which may be highly beneficial in treating keloids.<\/jats:p>","DOI":"10.1111\/exd.12719","type":"journal-article","created":{"date-parts":[[2015,4,11]],"date-time":"2015-04-11T15:33:29Z","timestamp":1428766409000},"page":"591-597","source":"Crossref","is-referenced-by-count":36,"title":["Decorin\u2010expressing adenovirus decreases collagen synthesis and upregulates MMP expression in keloid fibroblasts and keloid spheroids"],"prefix":"10.1111","volume":"24","author":[{"given":"Won Jai","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery College of Medicine Institute for Human Tissue Restoration Yonsei University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyo Min","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Bioengineering College of 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